ATG3 Protein (AA 1-287) (His tag,T7 tag)
Quick Overview for ATG3 Protein (AA 1-287) (His tag,T7 tag) (ABIN1096065)
Target
See all ATG3 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 1-287
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Purification tag / Conjugate
- This ATG3 protein is labelled with His tag,T7 tag.
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Purpose
- Recombinant Human Autophagy Related 3 Homolog/ATG3 (C-6His, N-T7 tag)
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Sequence
- GSMQNVINTV KGKALEVAEY LTPVLKESKF KETGVITPEE FVAAGDHLVH HCPTWQWATG EELKVKAYLP TGKQFLVTKN VPCYKRCKQM EYSDELEAII EEDDGDGGWV DTYHNTGITG ITEAVKEITL ENKDNIRLQD CSALCEEEED EDEGEAADME EYEESGLLET DEATLDTRKI VEACKAKTDA GGEDAILQTR TYDLYITYDK YYQTPRLWLF GYDEQRQPLT VEHMYEDISQ DHVKKTVTIE NHPHLPPPPM CSVHPCRHAE VMKKIIETVA EGGGELGVHM YLLIFLKFVQ AVIPTIEYDY TRHFTM
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Characteristics
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Recombinant Human Autophagy Related 3 Homolog/ATG3 (C-6His, N-T7 tag)
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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ATG3 Autophagy Related 3 (ATG3) (AA 1-314) protein (His tag)
custom-made ATG3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
ATG3 Autophagy Related 3 (ATG3) (AA 1-314) protein (His tag)custom-made ATG3 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
ATG3 Autophagy Related 3 (ATG3) (AA 1-287) protein (His tag)ATG3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
ATG3 Autophagy Related 3 (ATG3) (AA 1-314) protein (GST tag)ATG3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
ATG3 Autophagy Related 3 (ATG3) (AA 1-314) protein (His tag)ATG3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % by SDS - PAGE SDS
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Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl,10 % Glycerol, pH 8.0.
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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Storage
- -80 °C
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Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. -
Expiry Date
- 6 months
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- ATG3 (ATG3 Autophagy Related 3 (ATG3))
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Alternative Name
- Ubiquitin-Like-Conjugating Enzyme ATG3/ATG3
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Background
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Recombinant Human Ubiquitin-Like-Conjugating Enzyme ATG3/ATG3 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-His287) of Human ATG3 fused with a 6His tag at the C-terminus.
Ubiquitin-Like-Conjugating Enzyme ATG3 (ATG3) is widely expressed and has highly levels in heart, skeletal muscle, kidney, liver and placenta. ATG3 as a E2-like enzyme, involves in autophagy and mitochondrial homeostasis. ATG3 catalyzes the conjugation of ATG8-like proteins to PE which is essential for autophagy. As an autocatalytic E2-like enzyme, ATG3 also can catalyzes the conjugation of ATG12 to itself which palys a role in mitochondrial homeostasis but not in autophagy. -
Molecular Weight
- 36 kDa
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UniProt
- Q9NT62
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Pathways
- Autophagy
Target
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