CRP Protein (AA 19-224) (His tag)

Catalog No. ABIN1096162

This Recombinant CRP protein is expressed in Escherichia coli (E. coli).

Quick Overview for CRP Protein (AA 19-224) (His tag) (ABIN1096162)

Target: CRP (C-Reactive Protein (CRP))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 19-224

Purification tag / Conjugate: This CRP protein is labelled with His tag.

Purpose: Recombinant Human C-Reactive Protein/CRP (C-6His)

Sequence: MQTDMSRKAF VFPKESDTSY VSLKAPLTKP LKAFTVCLHF YTELSSTRGY SIFSYATKRQ DNEILIFWSK DIGYSFTVGG SEILFEVPEV TVAPVHICTS WESASGIVEF WVDGKPRVRK SLKKGYTVGA EASIILGQEQ DSFGGNFEGS QSLVGDIGNV NMWDFVLSPD EINTIYLGGP FSPNVLNWRA LKYEVQGEVF TKPQLWPLEH HHHHH

Characteristics: Recombinant Human C-Reactive Protein/CRP is produced by our E. coli expression system. The target protein is expressed with sequence (Q19-P224) of Human CRP.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mMTris,2MUrea, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: CRP (C-Reactive Protein (CRP))

Alternative Name: C-Reactive-Protein

Background: C-reactive protein (CRP) belongs to the pentaxin family. CRP is a secreted protein found in plasma. It can binds two calcium ions per subunit.CRP can promotes phagocytosis, bacterial capsular swelling, complement fixation and agglutination through its calcium-dependent binding to phosphorylcholine which expressed on the surface of dead or dying cells. It can activate the complement system via the C1Q complex. CRP can interact with DNA and histones. In addition, CRP may scavenge nuclear material released from damaged circulating cells.
Alternative Names: C-Reactive Protein, CRP, PTX1

Molecular Weight: 23.04 kDa

UniProt: P02741

Pathways: Carbohydrate Homeostasis