CRISP3 Protein (AA 21-245) (His tag)

Catalog No. ABIN1096446

This Recombinant CRISP3 protein is expressed in Human Cells.

Quick Overview for CRISP3 Protein (AA 21-245) (His tag) (ABIN1096446)

Target: CRISP3 (Cysteine-Rich Secretory Protein 3 (CRISP3))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 21-245

Purification tag / Conjugate: This CRISP3 protein is labelled with His tag.

Purpose: Recombinant Human Cysteine-Rich Secretory Protein 3/CRISP-3/SGP28 (C-6His)

Sequence: NEDKDPAFTA LLTTQTQVQR EIVNKHNELR RAVSPPARNM LKMEWNKEAA ANAQKWANQC NYRHSNPKDR MTSLKCGENL YMSSASSSWS QAIQSWFDEY NDFDFGVGPK TPNAVVGHYT QVVWYSSYLV GCGNAYCPNQ KVLKYYYVCQ YCPAGNWANR LYVPYEQGAP CASCPDNCDD GLCTNGCKYE DLYSNCKSLK LTLTCKHQLV RDSCKASCNC SNSIYVDHHH HHH

Characteristics: Recombinant Human Cysteine-Rich Secretory Protein 3/CRISP-3 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (N21-Y245) of Human CRISP-3 fused with a polyhistidine tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: CRISP3 (Cysteine-Rich Secretory Protein 3 (CRISP3))

Alternative Name: crisp-3

Sub Type: Fusionprotein

Background: Cysteine-rich secretory protein 3 (CRISP-3) is a secreted protein,containing 1 SCP domain and 1 ShKT domain.It is belongs to the CRISP family. CRISP-3 is a glycoprotein that belongs to the family of cysteine-rich secretory proteins (CRISPs) which was originally discovered in human neutrophilic granulocytes. CRISP-3 is also widely distibuted in exocrine glands (salivary glands, pancreas and prostate), eosinophilic granulocytes and to a lower level in epididymis, ovary, thymus and colon. The presence of CRISP-3 in neutrophils, eosinophils and in exocrine secretions indicates a role in innate host defense. The antibody has been raised against recombinant C-terminally truncated form of CRISP-3 and recognizes both the N-glycosylated and non-glycosylated form of the mature protein.
Alternative Names: Cysteine-Rich Secretory Protein 3, CRISP-3, Specific Granule Protein of 28 kDa, SGP28, CRISP3

Molecular Weight: 26.54 kDa

UniProt: P54108