PPIC Protein (AA 31-182) (His-TRX)

Catalog No. ABIN1096479

This Recombinant PPIC protein is produced in Escherichia coli (E. coli).

Quick Overview for PPIC Protein (AA 31-182) (His-TRX) (ABIN1096479)

Target: PPIC (Peptidylprolyl Isomerase C (Cyclophilin C) (PPIC))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 31-182

Purification tag / Conjugate: This PPIC protein is labelled with His-TRX.

Purpose: Recombinant Human Cyclophilin C/PPIase C/PPIC (N-Trx, 6His)

Sequence: MSDKIIHLTD DSFDTDVLKA DGAILVDFWA EWCGPCKMIA PILDEIADEY QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL LFKNGEVAAT KVGALSKGQL KEFLDANLAG SGSGHMHHHH HHSSGLVPRG SGMKETAAAK FERQHMDSPD LGTDDDDKAM AKRGPSVTAK VFFDVRIGDK DVGRIVIGLF GKVVPKTVEN FVALATGEKG YGYKGSKFHR VIKDFMIQGG DITTGDGTGG VSIYGETFPD ENFKLKHYGI GWVSMANAGP DTNGSQFFIT LTKPTWLDGK HVVFGKVIDG MTVVHSIELQ ATD

Characteristics: Recombinant Human Peptidyl-Prolyl cis-trans Isomerase C is produced by our E.coli expression system and the target gene encoding Lys31-Asp182 is expressed with a Trx, 6His tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 um filtered solution of 20 mM PB, 150 mM NaCl, 10 % Glycerol, pH 7.4.

Handling Advice: minimize freeze-thaw cycles

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: PPIC (Peptidylprolyl Isomerase C (Cyclophilin C) (PPIC))

Alternative Name: Cyclophilin C

Background: Alternative Names: Peptidyl-Prolyl Cis-Trans Isomerase C, PPIase C, Cyclophilin C, Rotamase C, PPIC, CYPCRecombinant Human Cyclophilin C/CYPC is produced by our E. coli expression system. The target protein is expressed with sequence (Trp131-Asp182) of Human CYPC fused with a His tag at the N-terminus.
Cyclophilin C is an enzyme (EC 5.2.1.8) found in both prokaryotes and eukaryotes that interconverts the cis and trans isomers of peptide bonds with the AA proline. Proline has an unusually conformationally restrained peptide bond due to its cyclic structure with its side chain bonded to its secondary amine nitrogen. Most AAs have a strong energetic preference for the trans peptide bond conformation due to steric hindrance, but prolines unusual structure stabilizes the cis form so that both isomers are populated under biologically relevant conditions. Proteins with prolyl isomerase activity include cyclophilin, FKBPs, and parvulin, although larger proteins can also contain prolyl isomerase domains.

Molecular Weight: 33.78 kDa

UniProt: P45877