POLB Protein (AA 2-335) (His tag)
Quick Overview for POLB Protein (AA 2-335) (His tag) (ABIN1096540)
Target
See all POLB ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 2-335
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Purification tag / Conjugate
- This POLB protein is labelled with His tag.
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Purpose
- Recombinant Human DNA Polymerase β (C-6His)
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Sequence
- MSKRKAPQET LNGGITDMLT ELANFEKNVS QAIHKYNAYR KAASVIAKYP HKIKSGAEAK KLPGVGTKIA EKIDEFLATG KLRKLEKIRQ DDTSSSINFL TRVSGIGPSA ARKFVDEGIK TLEDLRKNED KLNHHQRIGL KYFGDFEKRI PREEMLQMQD IVLNEVKKVD SEYIATVCGS FRRGAESSGD MDVLLTHPSF TSESTKQPKL LHQVVEQLQK VHFITDTLSK GETKFMGVCQ LPSKNDEKEY PHRRIDIRLI PKDQYYCGVL YFTGSDIFNK NMRAHALEKG FTINEYTIRP LGVTGVAGEP LPVDSEKDIF DYIQWKYREP KDRSEVEHHH HHH
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Characteristics
- Recombinant Human DNA Polymerase beta is produced with our E. coli expression system. The target protein is expressed with sequence (Ser2-Glu335) of Human DNA Polymerase beta.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Polymerase (DNA Directed), beta (POLB) (AA 1-335) protein (His tag)
custom-made POLB Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Polymerase (DNA Directed), beta (POLB) (AA 1-335) protein (His tag)custom-made POLB Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Polymerase (DNA Directed), beta (POLB) (AA 2-335) protein (His tag)POLB Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Polymerase (DNA Directed), beta (POLB) (AA 1-335) protein (His tag)POLB Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Polymerase (DNA Directed), beta (POLB) (AA 1-335) protein (GST tag)POLB Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 1 mM DTT, 1 mM EDTA, 50 % Glycerol, pH 7.8.
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Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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Storage
- -80 °C
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Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. -
Expiry Date
- 6 months
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- POLB (Polymerase (DNA Directed), beta (POLB))
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Alternative Name
- DNA Polymerase beta
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Background
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Human DNA polymerase beta is constitutively expressed in cells. It fills in gaps in DNA that are formed following base excision repair. The activity cannot be affected by Aphidicolin, which is an inhibitor of DNA polymerase beta.
Alternative Names: DNA Polymerase Beta, POLB -
Molecular Weight
- 39.23 kDa
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UniProt
- P06746
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Pathways
- DNA Damage Repair
Target
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