ESM1 Protein (AA 20-184) (His tag)

Catalog No. ABIN1096566

Recombinant ESM1 protein expressed in Human Cells.

Quick Overview for ESM1 Protein (AA 20-184) (His tag) (ABIN1096566)

Target: ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 20-184

Purification tag / Conjugate: This ESM1 protein is labelled with His tag.

Purpose: Recombinant Human Endocan/ESM-1 (C-6His)

Sequence: WSNNYAVDCP QHCDSSECKS SPRCERTVLD DCGCCRVCAA GRGETCYRTV SGMDGMKCGP GLRCQPSNGE DPFGEEFGIC KDCPYGTFGM DCRETCNCQS GICDRGTGKC LKFPFFQYSV TKSSNRFVSL TEHDMASGDG NIVREEVVKE NAAGSPVMRK WLNPRVDHHH HHH

Characteristics: Recombinant Human Endothelial Cell-Specific Molecule 1/ESM-1 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (W20-R184) of Human ESM1 fused with a polyhistidine tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))

Alternative Name: endocan

Sub Type: Fusionprotein

Background: ESM-1, short from Endothelial cell-specific molecule 1, is expressed in lung, on the vascular capillary network within alveolar walls, and also at lower level in kidney. It is a secretory protein, and can be inducted by TNF and IL1B/interleukin-1beta, but not IL4/interleukin-4. This protein plays great roles in angiogenesis, sprouting, and may have potent implications in lung endothelial cell-leukocyte interactions.
Alternative Names: Endothelial Cell-Specific Molecule 1, ESM-1, ESM1

Molecular Weight: 19.16 kDa

UniProt: Q9NQ30

Pathways: Growth Factor Binding