LGALS1/Galectin 1 Protein (AA 2-135) (His tag)

Catalog No. ABIN1096714

This Recombinant LGALS1/Galectin 1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for LGALS1/Galectin 1 Protein (AA 2-135) (His tag) (ABIN1096714)

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 2-135

Purification tag / Conjugate: This LGALS1/Galectin 1 protein is labelled with His tag.

Purpose: Recombinant Human Galectin-1/LGALS1 (C-6His)

Sequence: ACGLVASNLN LKPGECLRVR GEVAPDAKSF VLNLGKDSNN LCLHFNPRFN AHGDANTIVC NSKDGGAWGT EQREAVFPFQ PGSVAEVCIT FDQANLTVKL PDGYEFKFPN RLNLEAINYM AADGDFKIKC VAFDLEHHHH HH

Characteristics: Recombinant Human Galectin-1 is produced by our E. coli expression system. The target protein is expressed with sequence (Ala2-Asp135) of Human LGALS1 fused with a 6His tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Comment:

Biological activity: Measured by its ability to agglutinate human red blood cells.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 10 mM PB, 200 mM NaCl, 2 mM DTT, pH 7.0.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Alternative Name: Galectin-1

Sub Type: Fusionprotein

Background: Galectin-1 is a member of growing family of evolutionary conserved animal lectins. Galectin-1 is widely expressed in many cells and tissues. Galectins consists of a Galectin domain and two Beta-galactoside binding domains. Galectin-1 can binds LGALS3BP and interacts with CD2, CD3, CD4, CD7, CD43 and CD45. Galectin-1 may act as an autocrine negative growth factor which regulates apoptosis, cell proliferation and cell differentiation. In addition, Galectin-1 plays improtant roles in immunosuppressive and antiinflammatory properties.
Alternative Names: Galectin-1, Gal-1, 14 kDa Laminin-Binding Protein, HLBP14, 14 kDa Lectin, Beta-Galactoside-Binding Lectin L-14-I, Galaptin, HBL, HPL, Lactose-Binding Lectin 1, Lectin Galactoside-Binding Soluble 1, Putative MAPK-Activating Protein PM12, S-Lac Lectin 1, LG

Molecular Weight: 15.78 kDa

UniProt: P09382

Pathways: Carbohydrate Homeostasis