HPD Protein (AA 1-393) (His tag)

Catalog No. ABIN1096834

Product Details

Target: HPD (4-Hydroxyphenylpyruvate Dioxygenase (HPD))

Protein Type: Recombinant

Protein Characteristics: AA 1-393

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This HPD protein is labelled with His tag.

Purpose: Recombinant Human 4-Hydroxyphenylpyruvate Dioxygenase/4HPPD/HPPDase (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MTTYSDKGAK PERGRFLHFH SVTFWVGNAK QAASFYCSKM GFEPLAYRGL ETGSREVVSH VIKQGKIVFV LSSALNPWNK EMGDHLVKHG DGVKDIAFEV EDCDYIVQKA RERGAKIMRE PWVEQDKFGK VKFAVLQTYG DTTHTLVEKM NYIGQFLPGY EAPAFMDPLL PKLPKCSLEM IDHIVGNQPD QEMVSASEWY LKNLQFHRFW SVDDTQVHTE YSSLRSIVVA NYEESIKMPI NEPAPGKKKS QIQEYVDYNG GAGVQHIALK TEDIITAIRH LRERGLEFLS VPSTYYKQLR EKLKTAKIKV KENIDALEEL KILVDYDEKG YLLQIFTKPV QDRPTLFLEV IQRHNHQGFG AGNFNSLFKA FEEEQNLRGN LTNMETNGVV PGM

Characteristics: Recombinant Human 4-Hydroxyphenylpyruvate Dioxygenase/4HPPD is produced with our E. coli expression system. The target protein is expressed with sequence (Thr2-Met393) of Human HPD fused with a 6His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 50 mM NaCl, 1 mM DTT, 0.1 mM PMSF, pH 8.0.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: HPD (4-Hydroxyphenylpyruvate Dioxygenase (HPD))

Alternative Name: HPD (HPD Products)

Synonyms: fb58f02 Protein, hpd Protein, wu:fb58f02 Protein, zgc:56326 Protein, zgc:92456 Protein, MGC146689 Protein, BA0240 Protein, PSPTO3553 Protein, DDBDRAFT_0203373 Protein, DDBDRAFT_0231603 Protein, DDBDRAFT_0231604 Protein, DDB_0203373 Protein, DDB_0231603 Protein, DDB_0231604 Protein, hppd Protein, 4-HPPD Protein, 4HPPD Protein, GLOD3 Protein, HPPDASE Protein, PPD Protein, 4-HYDROXYPHENYLPYRUVATE DIOXYGENASE Protein, F12K11.9 Protein, F12K11_9 Protein, HPD Protein, P-HYDROXYPHENYLPYRUVATE DIOXYGENASE Protein, phytoene desaturation 1 Protein, Fla Protein, Flp Protein, Hppd Protein, Laf Protein, 4-hydroxyphenylpyruvate dioxygenase a Protein, 4-hydroxyphenylpyruvate dioxygenase Protein, 4-hydroxyphenylpyruvate dioxygenase b Protein, 4-hydroxyphenylpyruvate dioxygenase L homeolog Protein, 4-hydroxyphenylpyruvic acid dioxygenase Protein, hpda Protein, HPD Protein, hpdb Protein, hpd.L Protein, hpd Protein, BA_0240 Protein, hppD Protein, Hpd Protein, PDS1 Protein

Sub Type: Fusionprotein

Background: 4-Hydroxyphenylpyruvate Dioxygenase (4HPPD) belongs to the 4HPPD family. 4HPPD is a key enzyme in the degradation of tyrosine, which catalyzes the second reaction in the catabolism of tyrosine the conversation of 4-hydroxyphenylpyruvate to homogentisate. 4HPPD exists in homodimer forms, which uses zinc as a cofactor to catalyze the third step in the conversion of L-phenylalanine to fumarate and acetoacetic acid. When the active 4HPPD enzyme concentration is low in the human body, it results in high levels of tyrosine concentration in the blood, which can cause mild mental retardation at birth, and degradation in vision as a patient grows older.
Alternative Names: 4-Hydroxyphenylpyruvate Dioxygenase, 4-Hydroxyphenylpyruvic Acid Oxidase, 4HPPD, HPD, HPPDase, HPD, PPD

Molecular Weight: 47.1 kDa

UniProt: P32754