IFN alpha 2a (Lys46) (Active) Protein
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
> 95 % as determined by reducing SDS-PAGE.
Func
Active
-
- Target
- IFN alpha 2a
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- Lys46
- Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Application
- Functional Studies (Func)
- Purpose
- Recombinant Human Interferon α2A Variant (Lys46)/IFN-α2A
- Sequence
- MCDLPQTHSL GSRRTLMLLA QMRKISLFSC LKDRHDFGFP QEEFGNQFQK AETIPVLHEM IQQIFNLFST KDSSAAWDET LLDKFYTELY QQLNDLEACV IQGVGVTETP LMKEDSILAV RKYFQRITLY LKEKKYSPCA WEVVRAEIMR SFSLSTNLQE SLRSKE
- Characteristics
- Recombinant Human Interferon-alpha2A/IFN-alpha2A produced in E. coli is a single non-glycosylated polypeptide chain containing 165 amino acids with a molecular mass of 19,241 Daltons.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
-
IFN alpha 2a protein
Origin: Human Recombinant CC, WB
-
- Comment
-
Biological activity: Specific Activity is greater than 1.0 x 108 IU/ mg as determined in a viral resistance assay using VSV-WISH cells.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
-
It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
-
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - Expiry Date
- 3 months
-
- Target
- IFN alpha 2a
- Alternative Name
- IFN-alpha 2a
- Background
-
At least 23 different variants of IFN-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes only differ in their sequences by one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.
Alternative Names: Interferon Alpha-2, IFN-Alpha-2, Interferon Alpha-A, LeIF A, IFNA2 - Molecular Weight
- 19.24 kDa
- UniProt
- P01563
-
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