IFN alpha 2a (Active) Protein

Catalog No. ABIN987977

Product Details

Target: IFN alpha 2a

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Sequence: analysis The sequence of the first five N-terminal amino acids was determined and was found to be Met-Cys-Asp-Leu-Pro, conforming to the sequence of native human IFN-alpha. N-terminal methionine has been completely removed enzymatica lly. p to grams level.

Characteristics: The specific activity as determined in a viral resistance assay using VSV-WISH cells was found to be greater than 1.0 x 108 IU/ mg.

Purity: > 98.0 % as determined by both:(a) Analysis by RP-HPLC.(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Endotoxin Level: Level Less than 0.1 ng/µg (IEU/µg) of recombinant human Interferon alpha 2a (IFN alpha 2a

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended to reconstitute the lyophilized Recombinant Human Interferon alpha 2a (IFN alpha 2a) in sterile 18 M-omega-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. Quantitation Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using a calibrated solution of IFN-alpha 2a as a Reference Standard.

Storage: -20 °C

References

Da Silva-Ferrada, Xolalpa, Lang, Aillet, Martin-Ruiz, de la Cruz-Herrera, Lopitz-Otsoa, Carracedo, Goldenberg, Rivas, England, Rodríguez: "Analysis of SUMOylated proteins using SUMO-traps." in: Scientific reports, Vol. 3, pp. 1690, (2013) (PubMed).

Target Details

Target: IFN alpha 2a

Alternative Name: IFN-alpha 2a

Background: Human Interferon alpha 2a (IFN alpha 2a) produced in E. coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19,241 Da. The Interferon alpha 2a (IFN alpha 2a) gene was obtained from human leukocytes. The Recombinant Human Interferon alpha 2a (IFN alpha 2a) is purified by proprietary chromatographic techniques. Synonym: rHuIFN-alpha 2a, rHuIFNalpha 2a, rHuIFN-a 2a, rHuIFN-alfa 2a, rHuIFNalfa 2a, Interferon-alpha 2a, Interferonalpha 2a, Interferon-a 2a, Interferon-alfa 2a, Interferonalfa 2. Formulation: Lyophilized from (1 mg/ml) solution containing 7.21 sodium chloride and 0.77 mg ammonium acetate.

Molecular Weight: 19,241 Da