MAX Protein (AA 1-151) (His tag)

Catalog No. ABIN1097137

Recombinant MAX protein expressed in Escherichia coli (E. coli).

Quick Overview for MAX Protein (AA 1-151) (His tag) (ABIN1097137)

Target: MAX (MYC Associated Factor X (MAX))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-151

Purification tag / Conjugate: This MAX protein is labelled with His tag.

Purpose: Recombinant Human Myc-Associated Factor X/MAX (C-6His)

Sequence: MSDNDDIEVE SDADKRAHHN ALERKRRDHI KDSFHSLRDS VPSLQGEKAS RAQILDKATE YIQYMRRKNH THQQDIDDLK RQNALLEQQV RALEKARSSA QLQTNYPSSD NSLYTNAKGS TISAFDGGSD SSSESEPEEP QSRKKLRMEA SLEHHHHHH

Characteristics: Recombinant Human Myc-Associated Factor X/MAX is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Ser151) of Human MAX.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 50 mM Imidazole, 250 mM NaCl, pH 8.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: MAX (MYC Associated Factor X (MAX))

Alternative Name: myc-associated-factor-x

Background: Myc-Associated Factor X (MAX) is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It contains 1 basic helix-loop-helix (bHLH) domain. It is found in the brain, heart, and lung at high levels while lower levels are seen in the liver, kidney, and skeletal muscle. MAX forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC-MAX complex is a transcriptional activator, whereas the MAD-MAX complex is a repressor. It may repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity.
Alternative Names: Protein Max, Class D Basic Helix-Loop-Helix Protein 4, bHLHd4, Myc-Associated Factor X, MAX, BHLHD4

Molecular Weight: 18.27 kDa

UniProt: P61244

Pathways: Mitotic G1-G1/S Phases