SENP8 Protein (AA 1-212) (His tag)
Quick Overview for SENP8 Protein (AA 1-212) (His tag) (ABIN1097213)
Target
See all SENP8 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 1-212
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Purification tag / Conjugate
- This SENP8 protein is labelled with His tag.
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Purpose
- Recombinant Human Sentrin-Specific Protease 8/SENP8 (N-6His)
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Sequence
- MGSSHHHHHH SSGLVPRGSH MDPVVLSYMD SLLRQSDVSL LDPPSWLNDH IIGFAFEYFA NSQFHDCSDH VSFISPEVTQ FIKCTSNPAE IAMFLEPLDL PNKRVVFLAI NDNSNQAAGG THWSLLVYLQ DKNSFFHYDS HSRSNSVHAK QVAEKLEAFL GRKGDKLAFV EEKAPAQQNS YDCGMYVICN TEALCQNFFR QQTESLLQLL TPAYITKKRG EWKDLIATLA KK
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Characteristics
- Recombinant Human Sentrin-Specific Protease 8/SENP8 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Lys212) of Human SENP8.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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SUMO/sentrin Specific Peptidase Family Member 8 (SENP8) (AA 1-212) protein (His tag)
custom-made SENP8 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
SUMO/sentrin Specific Peptidase Family Member 8 (SENP8) (AA 1-221) protein (His tag)custom-made SENP8 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
SUMO/sentrin Specific Peptidase Family Member 8 (SENP8) (AA 1-212) protein (His-IF2DI Tag)SENP8 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
SUMO/sentrin Specific Peptidase Family Member 8 (SENP8) (AA 1-212) protein (GST tag)SENP8 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
SUMO/sentrin Specific Peptidase Family Member 8 (SENP8) (AA 1-212), (full length) protein (His-SUMO Tag)SENP8 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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Storage
- -80 °C
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Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. -
Expiry Date
- 6 months
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- SENP8 (SUMO/sentrin Specific Peptidase Family Member 8 (SENP8))
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Alternative Name
- Sentrin-Specific Protease 8/SENP8
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Background
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Sentrin-Specific Protease 8 (SENP8) mediates the reversible covalent modification of proteins by NEDD8. SENP8 catalyzes the full-length NEDD8 to generate its mature form and deconjugation of NEDD8 from targeted proteins such as CUL2 , CUL4A in vivo, or p53. but it does not show activity against ubiquitin or SUMO proteins.
Alternative Names: Sentrin-Specific Protease 8, Deneddylase-1, NEDD8-Specific Protease 1, Protease Cysteine 2, Sentrin/SUMO-Specific Protease SENP8, SENP8, DEN1, NEDP1, PRSC2 -
Molecular Weight
- 26.24 kDa
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UniProt
- Q96LD8
Target
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