PKIB Protein (AA 1-78) (His tag)

Catalog No. ABIN1097341

This Recombinant PKIB protein is produced in Escherichia coli (E. coli).

Quick Overview for PKIB Protein (AA 1-78) (His tag) (ABIN1097341)

Target: PKIB (Protein Kinase (CAMP-Dependent, Catalytic) Inhibitor beta (PKIB))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-78

Purification tag / Conjugate: This PKIB protein is labelled with His tag.

Purpose: Recombinant Human cAMP-dependent Protein Kinase Inhibitor β/PKI-β (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MRTDSSKMTD VESGVANFAS SARAGRRNAL PDIQSSAATD GTSDLPLKLE ALSVKEDAKE KDEKTTQDQL EKPQNEEK

Characteristics:

Recombinant Human cAMP-dependent Protein Kinase Inhibitor β/PKI-β (N-6His)

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 1 mM DTT, 20 % Glycerol, pH 8.0.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: PKIB (Protein Kinase (CAMP-Dependent, Catalytic) Inhibitor beta (PKIB))

Alternative Name: PKI-beta

Background: Recombinant Human cAMP-dependent Protein Kinase Inhibitor β/PKI-β is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Lys78) of Human PKI-β fused with a His tag at the N-terminus.
cAMP-Dependent Protein Kinase Inhibitor β (PKI-β) is a member of the PKI family. It has been shown that PKI-β is an extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.

Molecular Weight: 10.6 kDa

UniProt: Q9C010

Pathways: Myometrial Relaxation and Contraction