PKIB Protein (AA 1-78) (His tag)
Quick Overview for PKIB Protein (AA 1-78) (His tag) (ABIN1097341)
Target
See all PKIB ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 1-78
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Purification tag / Conjugate
- This PKIB protein is labelled with His tag.
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Purpose
- Recombinant Human cAMP-dependent Protein Kinase Inhibitor β/PKI-β (N-6His)
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Sequence
- MGSSHHHHHH SSGLVPRGSH MRTDSSKMTD VESGVANFAS SARAGRRNAL PDIQSSAATD GTSDLPLKLE ALSVKEDAKE KDEKTTQDQL EKPQNEEK
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Characteristics
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Recombinant Human cAMP-dependent Protein Kinase Inhibitor β/PKI-β (N-6His)
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Protein Kinase (CAMP-Dependent, Catalytic) Inhibitor beta (PKIB) (AA 1-78) protein (Fc Tag)
custom-made PKIB Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Protein Kinase (CAMP-Dependent, Catalytic) Inhibitor beta (PKIB) (AA 1-92) protein (Fc Tag)custom-made PKIB Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Protein Kinase (CAMP-Dependent, Catalytic) Inhibitor beta (PKIB) (AA 1-98) protein (His-IF2DI Tag)PKIB Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Protein Kinase (CAMP-Dependent, Catalytic) Inhibitor beta (PKIB) (AA 1-78) protein (GST tag)PKIB Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Protein Kinase (CAMP-Dependent, Catalytic) Inhibitor beta (PKIB) (Transcript Variant 3) protein (His tag)PKIB Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 1 mM DTT, 20 % Glycerol, pH 8.0.
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Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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Storage
- -80 °C
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Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. -
Expiry Date
- 6 months
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- PKIB (Protein Kinase (CAMP-Dependent, Catalytic) Inhibitor beta (PKIB))
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Alternative Name
- PKI-beta
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Background
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Recombinant Human cAMP-dependent Protein Kinase Inhibitor β/PKI-β is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Lys78) of Human PKI-β fused with a His tag at the N-terminus.
cAMP-Dependent Protein Kinase Inhibitor β (PKI-β) is a member of the PKI family. It has been shown that PKI-β is an extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains. -
Molecular Weight
- 10.6 kDa
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UniProt
- Q9C010
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Pathways
- Myometrial Relaxation and Contraction
Target
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