PSPH Protein (AA 1-225) (His tag)
Quick Overview for PSPH Protein (AA 1-225) (His tag) (ABIN1097446)
Target
See all PSPH ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 1-225
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Purification tag / Conjugate
- This PSPH protein is labelled with His tag.
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Purpose
- Recombinant Human Phosphoserine Phosphatase/PSP (C-6His)
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Sequence
- MVSHSELRKL FYSADAVCFD VDSTVIREEG IDELAKICGV EDAVSEMTRR AMGGAVPFKA ALTERLALIQ PSREQVQRLI AEQPPHLTPG IRELVSRLQE RNVQVFLISG GFRSIVEHVA SKLNIPATNV FANRLKFYFN GEYAGFDETQ PTAESGGKGK VIKLLKEKFH FKKIIMIGDG ATDMEACPPA DAFIGFGGNV IRQQVKDNAK WYITDFVELL GELEELEHHH HHH
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Characteristics
- Recombinant Human Phosphoserine Phosphatase/PSP is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Glu225) of Human PSPH.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Phosphoserine Phosphatase (PSPH) (AA 1-225) protein (His tag)
custom-made PSPH Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Phosphoserine Phosphatase (PSPH) (AA 1-225) protein (His tag)custom-made PSPH Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Phosphoserine Phosphatase (PSPH) (AA 1-225) protein (GST tag)PSPH Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Phosphoserine Phosphatase (PSPH) (AA 1-382) protein (His tag)PSPH Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % pure SDS
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Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
Buffer
- Supplied as a 0.2 μm filtered solution of 50 mM Tris, 250 mM NaCl, 50 mM Imidazole, pH 8.5 .
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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Storage
- -80 °C
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Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. -
Expiry Date
- 6 months
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- PSPH (Phosphoserine Phosphatase (PSPH))
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Alternative Name
- PSPase
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Background
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Phosphoserine phosphatase (PSP) is an enzyme that belongs to the serB family. PSPH catalyzes magnesium-dependent hydrolysis of L-phosphoserine and is also involved in an exchange reaction between L-serine and L-phosphoserine. The reaction mechanism proceeds via the formation of a phosphoryl-enzyme intermediates. Deficiency of this protein is thought to be linked to Williams syndrome. A disorder that results in pre- and postnatal growth retardation, moderate psychomotor retardation and facial features suggestive of Williams syndrome.
Alternative Names: Phosphoserine Phosphatase, PSP, PSPase, L-3-Phosphoserine Phosphatase, O-Phosphoserine Phosphohydrolase, PSPH -
Molecular Weight
- 26.07 kDa
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UniProt
- P78330
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Pathways
- Warburg Effect
Target
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