SHP1 Protein (AA 243-541) (His tag)

Catalog No. ABIN1097451

This Recombinant SHP1 protein is produced in Escherichia coli (E. coli).

Quick Overview for SHP1 Protein (AA 243-541) (His tag) (ABIN1097451)

Target: SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 243-541

Purification tag / Conjugate: This SHP1 protein is labelled with His tag.

Purpose: Recombinant Human Protein-Tyrosine Phosphatase 1C/PTP1C (C-6His)

Sequence: MKAGFWEEFE SLQKQEVKNL HQRLEGQRPE NKGKNRYKNI LPFDHSRVIL QGRDSNIPGS DYINANYIKN QLLGPDENAK TYIASQGCLE ATVNDFWQMA WQENSRVIVM TTREVEKGRN KCVPYWPEVG MQRAYGPYSV TNCGEHDTTE YKLRTLQVSP LDNGDLIREI WHYQYLSWPD HGVPSEPGGV LSFLDQINQR QESLPHAGPI IVHCSAGIGR TGTIIVIDML MENISTKGLD CDIDIQKTIQ MVRAQRSGMV QTEAQYKFIY VAIAQFIETT KKKLEVLQSQ KGQESEYGNI LEHHHHHH

Characteristics: Recombinant Human Protein-Tyrosine Phosphatase 1C/PTP1C is produced with our E. coli expression system. The target protein is expressed with sequence (Lys243-Ile541) of Human PTP1C

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 25 mM TrisHCl, 2 mM ß-ME, 1 mM EDTA, 1 mM DTT, 20 % Glycerol, pH 7.5.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))

Alternative Name: Protein-Tyrosine Phosphatase 1C/PTP1C

Background: Protein-Tyrosine Phosphatase 1C (PTP1C) belongs to the protein-tyrosine phosphatase family.which is known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTP1C is highly expressed in leukocyte cell type. It contains two SH2 domains and one tyrosine-protein phosphatase domain. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. In addition, PTP1C also modulates signaling by tyrosine phosphorylated cell surface receptors.
Alternative Names: Tyrosine-Protein Phosphatase Non-Receptor Type 6, Hematopoietic Cell Protein-Tyrosine Phosphatase, Protein-Tyrosine Phosphatase 1C, PTP-1C, Protein-Tyrosine Phosphatase SHP-1, SH-PTP1, PTPN6, HCP, PTP1C

Molecular Weight: 35.38 kDa

UniProt: P29350

Pathways: JAK-STAT Signaling, TCR Signaling, TLR Signaling, Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, BCR Signaling