SUMF1 Protein (AA 34-374) (His tag)

Catalog No. ABIN1097598

This Recombinant SUMF1 protein is expressed in Human Cells.

Quick Overview for SUMF1 Protein (AA 34-374) (His tag) (ABIN1097598)

Target: SUMF1 (Sulfatase Modifying Factor 1 (SUMF1))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 34-374

Purification tag / Conjugate: This SUMF1 protein is labelled with His tag.

Purpose: Recombinant Human Sulfatase Modifying Factor 1/SUMF1 (C-6His)

Sequence: SQEAGTGAGA GSLAGSCGCG TPQRPGAHGS SAAAHRYSRE ANAPGPVPGE RQLAHSKMVP IPAGVFTMGT DDPQIKQDGE APARRVTIDA FYMDAYEVSN TEFEKFVNST GYLTEAEKFG DSFVFEGMLS EQVKTNIQQA VAAAPWWLPV KGANWRHPEG PDSTILHRPD HPVLHVSWND AVAYCTWAGK RLPTEAEWEY SCRGGLHNRL FPWGNKLQPK GQHYANIWQG EFPVTNTGED GFQGTAPVDA FPPNGYGLYN IVGNAWEWTS DWWTVHHSVE ETLNPKGPPS GKDRVKKGGS YMCHRSYCYR YRCAARSQNT PDSSASNLGF RCAADRLPTM DVDHHHHHH

Characteristics: Recombinant Human Sulfatase Modifying Factor 1/SUMF1 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Ser34-Asp374) of Human SUMF1 fused with a polyhistidine tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, 2 mM CaCl2, 10 % Glycerol, pH 7.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: SUMF1 (Sulfatase Modifying Factor 1 (SUMF1))

Alternative Name: sumf1

Sub Type: Fusionprotein

Background: Human Sulfatase Modifying Factor 1 (SUMF1) is a 42kDa protein. SUMF1 is a Ca2+-binging member of the sulfatase-modifying factor family. SUMF1 is a soluble ER lumenal glycoprotein, it converts inactive sulfatases into an active form by transforming a catalytic site cysteine into a formylglycine residue. In the ER, SUMF1 can exist as either a monomer, or a disulfide-linked homodimer or a heterodimer with SUMF2. Three splice isoforms are known.
Alternative Names: Sulfatase-Modifying Factor 1, C-Alpha-Formylglycine-Generating Enzyme 1, SUMF1, FGE

Molecular Weight: 38.27 kDa

UniProt: Q8NBK3