LTA Protein (AA 35-205)

Catalog No. ABIN1097673

Product Details

Target: LTA (Lymphotoxin-alpha (LTA))

Protein Type: Recombinant

Protein Characteristics: AA 35-205

Origin: Human

Source: Escherichia coli (E. coli)

Purpose: Recombinant Human Tumor Necrosis Factor β/TNFβ

Sequence: MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL

Characteristics: Recombinant Human Tumor Necrosis Factor beta/TNF-beta is produced by our E. coli expression system. The target protein is expressed with sequence (Leu35-Leu205) of Human TNF-beta.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: LTA (Lymphotoxin-alpha (LTA))

Alternative Name: tnf-beta (LTA Products)

Synonyms: LT Protein, TNFB Protein, TNFSF1 Protein, LT-[a] Protein, LT-alpha Protein, LT[a] Protein, LTalpha Protein, Ltx Protein, TNF-beta Protein, Tnfb Protein, Tnfsf1b Protein, hlb382 Protein, lymphotoxin alpha Protein, lymphotoxin A Protein, LTA Protein, Lta Protein

Target Type: Chemical

Background: Tumor Necrosis Factor beta (TNF-beta) is a secreted protein belonging to the tumor necrosis factor family. TNF-beta binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM in homotrimeric form, binds to TNFRSF3/LTBR in heterotrimeric form with LTB. TNF-beta forms heterotrimers with lymphotoxin-beta, which anchors TNF-beta to the cell surface. TNF-beta mediates the inflammatory, immunostimulatory, and antiviral response, involves in the formation of second lymphoid organs during development, has a role in apoptosis. TNF-beta is produced by lymphocytes and cytotoxic for a variety of tumor cells in vitro and in vivo.
Alternative Names: Lymphotoxin-Alpha, LT-Alpha, TNF-Beta, Tumor Necrosis Factor Ligand Superfamily Member 1, LTA, TNFB, TNFSF1

Molecular Weight: 18.8 kDa

UniProt: P01374

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process