CARD18 Protein (AA 1-90) (His tag)
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- Target See all CARD18 Proteins
- CARD18 (Caspase Recruitment Domain-Containing Protein 18 (ICEBERG) (CARD18))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-90
- Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This CARD18 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MADQLLRKKR RIFIHSVGAG TINALLDCLL EDEVISQEDM NKVRDENDTV MDKARVLIDL VTGKGPKSCC KFIKHLCEED PQLASKMGLH
- Purification
- purified by chromatography
- Purity
- > 95% by SDS-PAGE
- Top Product
- Discover our top product CARD18 Protein
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Caspase Recruitment Domain-Containing Protein 18 (ICEBERG) (CARD18) (AA 1-90) protein (His tag)
custom-made CARD18 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Caspase Recruitment Domain-Containing Protein 18 (ICEBERG) (CARD18) (AA 1-90) protein (His tag)CARD18 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Caspase Recruitment Domain-Containing Protein 18 (ICEBERG) (CARD18) (AA 1-90) protein (GST tag)CARD18 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Caspase Recruitment Domain-Containing Protein 18 (ICEBERG) (CARD18) protein (Myc-DYKDDDDK Tag)CARD18 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 1 MM DTT,10% glycerol, 0.1 M NaCl
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- Target
- CARD18 (Caspase Recruitment Domain-Containing Protein 18 (ICEBERG) (CARD18))
- Alternative Name
- CARD18 (CARD18 Products)
- Background
- Caspase recruitment domain-containing protein 18, also known as CARD18, is a member of the death-domain-fold superfamily that is induced by proinflammatory stimuli. It consists essentially only of a CARD, inhibits generation of IL-1b by interacting with caspase-1 and preventing association with RIP 2. The association of CARD18 with caspase-1 is facilitated by the charge-charge interactions between the prodomain of caspase-1 and the surface charge of CARD18. Recombinant human CARD18 proetin, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 12.3 kDa (110aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_067546
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