CARD18 Protein (AA 1-90) (His tag)

Catalog No. ABIN1098230

This Recombinant CARD18 protein is produced in Escherichia coli (E. coli).

Quick Overview for CARD18 Protein (AA 1-90) (His tag) (ABIN1098230)

Target: CARD18 (Caspase Recruitment Domain-Containing Protein 18 (ICEBERG) (CARD18))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95% by SDS-PAGE

Product Details

Protein Characteristics: AA 1-90

Purification tag / Conjugate: This CARD18 protein is labelled with His tag.

Sequence: MGSSHHHHHH SSGLVPRGSH MADQLLRKKR RIFIHSVGAG TINALLDCLL EDEVISQEDM NKVRDENDTV MDKARVLIDL VTGKGPKSCC KFIKHLCEED PQLASKMGLH

Purification: purified by chromatography

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 1 MM DTT,10% glycerol, 0.1 M NaCl

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: CARD18 (Caspase Recruitment Domain-Containing Protein 18 (ICEBERG) (CARD18))

Alternative Name: CARD18

Background: Caspase recruitment domain-containing protein 18, also known as CARD18, is a member of the death-domain-fold superfamily that is induced by proinflammatory stimuli. It consists essentially only of a CARD, inhibits generation of IL-1b by interacting with caspase-1 and preventing association with RIP 2. The association of CARD18 with caspase-1 is facilitated by the charge-charge interactions between the prodomain of caspase-1 and the surface charge of CARD18. Recombinant human CARD18 proetin, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 12.3 kDa (110aa) confirmed by MALDI-TOF

NCBI Accession: NP_067546