MDH protein (His tag)
Quick Overview for MDH protein (His tag) (ABIN1098249)
Target
Protein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- His tag
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Purification
- purified by chromatography
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MDH (AA 2-312) protein (His tag)
Origin: Bacillus subtilis Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
MDH (AA 1-312), (full length) protein (His tag)Origin: E. coli Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Comment
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Synonyms: malate dehydrogenase, ECK3225, JW3205
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/ml (determined by Bradford assay)
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Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 1 MM DTT, 10% glycerol, 50 mM NaCl
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Storage
- 4 °C
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Storage Comment
- Avoid repeated freezing and thawing cycles.
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- MDH
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Background
- Malate dehydrogenase (mdh) belongs to the LDH/MDH superfamily and MDH type 1 family. This enzyme catalyzes the conversion of malate into oxaloacetate (using NAD+) and vice versa (this is a reversible reaction). Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. Recombinant E.coli mdh protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 34.9kDa (336aa), confirmed by MALDI-TOF
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NCBI Accession
- NP_417703
Target
-
