IL12B Protein (AA 23-328) (His tag)

Catalog No. ABIN1098598

Product Details

Target: IL12B (Interleukin 12 beta (IL12B))

Protein Type: Recombinant

Protein Characteristics: AA 23-328

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This IL12B protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: IWELKKD VYVVELDWYP DAPGEMVVLT CDTPEEDGIT WTLDQSSEVL GSGKTLTIQV KEFGDAGQYT CHKGGEVLSH SLLLLHKKED GIWSTDILKD QKEPKNKTFL RCEAKNYSGR FTCWWLTTIS TDLTFSVKSS RGSSDPQGVT CGAATLSAER VRGDNKEYEY SVECQEDSAC PAAEESLPIE VMVDAVHKLK YENYTSSFFI RDIIKPDPPK NLQLKPLKNS RQVEVSWEYP DTWSTPHSYF SLTFCVQVQG KSKREKKDRV FTDKTSATVI CRKNASISVR AQDRYYSSSW SEWASVPCS

Purification: purified by using conventional chromatography.

Purity: > 90% by SDS-PAGE

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 2 mM DTT, 20% glycerol, 100 mM NaCl, 0.1 MM PMSF

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: IL12B (Interleukin 12 beta (IL12B))

Alternative Name: IL-12 p40 (IL12B Products)

Background: IL12P40 (interleukin 12 subunit beta), also known as natural killer cell stimulatory factor 2, or cytotoxic lymphocyte maturation factor 2, p40, belongs to the type I cytokine receptor family. This protein is a cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. Recombinant human IL12P40 protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell using baculovirus expression system and purified by using conventional chromatography.

Molecular Weight: 35.8 kDa (315aa)

NCBI Accession: NP_002178

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation