VEGF 165 (AA 207-371) Protein

Catalog No. ABIN1098802

Recombinant protein expressed in Escherichia coli (E. coli).

Quick Overview for VEGF 165 (AA 207-371) Protein (ABIN1098802)

Target: VEGF 165

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90% by SDS-PAGE

Product Details

Protein Characteristics: AA 207-371

Sequence: MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQENPCGP CSERRKHLFV QDPQTCKCSC KNTDSRCKAR QLELNERTCR CDKPRR

Purification: purified by chromatography

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 2 mM EDTA, 0.1 MM PMSF, 1 MM DTT

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: VEGF 165

Background: Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant human VEGF165 protein was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Molecular Weight: 19.3 kDa (166aa) confirmed by MALDI-TOF

NCBI Accession: NP_001020539