Insulin Protein (INS)
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- Target See all Insulin (INS) Proteins
- Insulin (INS)
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- Biochemical Assay (BCA)
- Characteristics
- DNA sequence encoding Human Insulin mature chain was expressed in Escherichia Coli.
- Purification
- >95%, as determined by SDS-PAGE and HPLC
- Purity
- >95%
- Endotoxin Level
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug).
- Biological Activity Comment
- Activity was determined to be 30IU/mg.
- Top Product
- Discover our top product INS Protein
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Insulin (INS) protein
INS Origin: Human Host: Yeast Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Insulin (INS) (AA 1-110) protein (GST tag)INS Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Insulin (INS) (AA 25-54), (AA 90-110) (Active) protein (His tag)INS Origin: Mouse Host: Escherichia coli (E. coli) > 97 % AcA, CC Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
- Preservative
- Without preservative
- Storage Comment
- The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
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- Target
- Insulin (INS)
- Alternative Name
- Insulin (INS Products)
- UniProt
- P01308
- Pathways
- NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
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