Insulin Protein (INS)

Catalog No. ABIN1111664

Recombinant Insulin protein expressed in Escherichia coli (E. coli).

Quick Overview for Insulin Protein (INS) (ABIN1111664)

Target: Insulin (INS)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Biochemical Assay (BCA)

Purity: >95%

Product Details

Characteristics: DNA sequence encoding Human Insulin mature chain was expressed in Escherichia Coli.

Purification: >95%, as determined by SDS-PAGE and HPLC

Endotoxin Level: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug).

Biological Activity Comment: Activity was determined to be 30IU/mg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Preservative: Without preservative

Storage Comment: The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Target Details

Target: Insulin (INS)

Alternative Name: Insulin

UniProt: P01308

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation