HMGB2 Protein

Catalog No. ABIN1176832

This Recombinant HMGB2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for HMGB2 Protein (ABIN1176832)

Target: HMGB2 (High Mobility Group Box 2 (HMGB2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activation (Act), Cellular Assay (CA)

Purity: > 95 % It contains no nucleic acids.

Product Details

Purpose: HMGB2 human, LPS-free

Sequence: HMGB2 consists of 195 amino acids: MGKGDPNKPR GKMSSYAFFV QTCREEHKKK HPDSSVNFAE FSKKCSERWK TMSAKEKSKF EDMAKSDKAR YDREMKNYVP PKGDKKGKKK DPNAPKRPPS AFFLFCSEHR PKIKSEHPGL SIGDTAKKLG EMWSEQSAKD KQPYEQKAAK LKEKYEKDIA AYRAKGKSEA GKKGPGRPTG SKKKNEPEDE EEEEE

Characteristics: The HMGB2 protein is the natural protein, with no tags or additional amino acids. The sequence of this product is that of Homo sapiens. It contains only trace amounts of LPS (<0.4 ng/mg protein) and it is tested for the ability to induce fibroblast migration. This product has been tested for activity in a cell migration assay. Maximal activity in the cell migration assay is obtained at 1 nM, or 25 ng/ml.

Purification: Purified. To avoid cysteine oxidation DTT 0.5 mM is added during protein purification.

Endotoxin Level: LPS free: Pierce™ Chromogenic Endotoxin Quant Kit, <0.1 EU/mL

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: The lyophilized protein once reconstituted will be dissolved in a solution containing 50 mM HEPES pH 7.9, 500 mM NaCl, DTT 0,5 mM.

Buffer: The protein is lyophilized from 50 mM HEPES buffer pH 7.9, 500 mM NaCl, 0.5 mM DTT
The product contains contains < 0.006% v/v of Triton X-114 due to LPS removal. Remaining traces can be removed upon request.

Handling Advice: The product is provided frozen. Repeated freezing and thawing is not recommended.

Storage: -20 °C

Storage Comment: 2-8°C when lyophilized. The protein once reconstituted with water can be stored frozen (-20°C).

Target Details

Target: HMGB2 (High Mobility Group Box 2 (HMGB2))

Alternative Name: High-mobility group protein B2 (HMGB2)

Background: HMGB2 is 80% identical to HMGB1 protein, but is encoded by a different gene. It consists of 195 amino acids and has a calculated molecular mass of approximately 22.3 kDa.

Molecular Weight: 22.3 kDa

Pathways: Cellular Response to Molecule of Bacterial Origin