beta-2 Microglobulin Protein

Catalog No. ABIN1305114

This Recombinant beta-2 Microglobulin protein is expressed in Insect Cells and has been mentioned in 2+ publications.

Quick Overview for beta-2 Microglobulin Protein (ABIN1305114)

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Protein Type: Recombinant

Origin: Human

Source: Insect Cells

Application: Functional Studies (Func)

Product Details

Brand: BD Pharmingen™

Purification: Human β2 Microglobulin recombinant protein was expressed in insect cells. The expressed protein was purified by a combination of ion-exchange and gel-filtration methods.

Application Details

Comment:

BD Pharmingen™ Recombinant Human β2 Microglobulin

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The major histocompatibility complex (MHC) gene locus encodes a group of highly polymorphic, cell surface proteins that play a broad role in the immune response to protein antigens. MHC molecules function by binding and presenting small antigenic protein fragments to antigen-specific receptors expressed by T cells (TCR). Class I MHC molecules consist of two separate polypeptide chains. The class I α chain is an MHC encoded, transmembrane polypeptide containing three extracellular domains: α1, α2 and α3. The second chain consists of a non-MHC encoded, 12 kD polypeptide called β2 microglobulin (β2M). Since β2M does not contain a transmembrane domain, it associates with the a chain through non-covalent interaction. This association is important for the stability of the MHC class I structure, its peptide-loading and its ability to present peptide antigen to CD8+ T cells. β2M is relatively invariant within each species. For example, human β2M is reported to have high affinity for human and mouse MHC class I heavy chains.

Storage: 4 °C

Storage Comment: Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.

References

Shields, Moffat, Ribaudo: "Functional comparison of bovine, murine, and human beta2-microglobulin: interactions with murine MHC I molecules." in: Molecular immunology, Vol. 35, Issue 14-15, pp. 919-28, (1999) (PubMed).

Silver, Parker, Wiley: "Reconstitution by MHC-restricted peptides of HLA-A2 heavy chain with beta 2-microglobulin, in vitro." in: Nature, Vol. 350, Issue 6319, pp. 619-22, (1991) (PubMed).

Target Details

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Alternative Name: beta 2 microglobulin

Background: The major histocompatibility complex (MHC) gene locus encodes a group of highly polymorphic, cell surface proteins that play a broad role in the immune response to protein antigens. MHC molecules function by binding and presenting small antigenic protein fragments to antigen-specific receptors expressed by T cells (TCR). Class I MHC molecules consist of two separate polypeptide chains. The class I α chain is an MHC encoded, transmembrane polypeptide containing three extracellular domains: α1, α2 and α3. The second chain consists of a non-MHC encoded, 12 kD polypeptide called β2 microglobulin (β2M). Since β2M does not contain a transmembrane domain, it associates with the a chain through non-covalent interaction. This association is important for the stability of the MHC class I structure, its peptide-loading and its ability to present peptide antigen to CD8+ T cells. β2M is relatively invariant within each species. For example, human β2M is reported to have high affinity for human and mouse MHC class I heavy chains.

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process