HDAC3 Protein (AA 1-428) (GST tag)

Catalog No. ABIN1306448

This Recombinant HDAC3 protein is expressed in Wheat germ and has been mentioned in 1 publication.

Quick Overview for HDAC3 Protein (AA 1-428) (GST tag) (ABIN1306448)

Target: HDAC3 (Histone Deacetylase 3 (HDAC3))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: ELISA, Western Blotting (WB), Affinity Purification (AP), Antibody Array (AA)

Product Details

Protein Characteristics: AA 1-428

Purification tag / Conjugate: This HDAC3 protein is labelled with GST tag.

Purpose: HDAC3 (Human) Recombinant Protein (P01)

Sequence: MAKTVAYFYD PDVGNFHYGA GHPMKPHRLA LTHSLVLHYG LYKKMIVFKP YQASQHDMCR FHSEDYIDFL QRVSPTNMQG FTKSLNAFNV GDDCPVFPGL FEFCSRYTGA SLQGATQLNN KICDIAINWA GGLHHAKKFE ASGFCYVNDI VIGILELLKY HPRVLYIDID IHHGDGVQEA FYLTDRVMTV SFHKYGNYFF PGTGDMYEVG AESGRYYCLN VPLRDGIDDQ SYKHLFQPVI NQVVDFYQPT CIVLQCGADS LGCDRLGCFN LSIRGHGECV EYVKSFNIPL LVLGGGGYTV RNVARCWTYE TSLLVEEAIS EELPYSEYFE YFAPDFTLHP DVSTRIENQN SRQYLDQIRQ TIFENLKMLN HAPSVQIHDV PADLLTYDRT DEADAEERGP EENYSRPEAP NEFYDGDHDN DKESDVEI

Characteristics: Human HDAC3 full-length ORF ( AAH00614, 1 a.a. - 428 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Jiang, Ye, Guo, Lu, Gao: "Inhibition of HDAC3 promotes ligand-independent PPAR? activation by protein acetylation." in: Journal of molecular endocrinology, Vol. 53, Issue 2, pp. 191-200, (2014) (PubMed).

Target Details

Target: HDAC3 (Histone Deacetylase 3 (HDAC3))

Alternative Name: HDAC3

Background: Full Gene Name: histone deacetylase 3
Synonyms: HD3,RPD3,RPD3-2

Gene ID: 8841

Pathways: Neurotrophin Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development