POLE Protein (AA 1-370) (GST tag)

Catalog No. ABIN1315634

This Recombinant POLE protein is produced in Wheat germ.

Quick Overview for POLE Protein (AA 1-370) (GST tag) (ABIN1315634)

Target: POLE (Polymerase (DNA Directed), Epsilon, Catalytic Subunit (POLE))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: Affinity Purification (AP), Antibody Array (AA), ELISA, Western Blotting (WB)

Product Details

Protein Characteristics: AA 1-370

Purification tag / Conjugate: This POLE protein is labelled with GST tag.

Purpose: POLE (Human) Recombinant Protein (P01)

Sequence: MDPSNYGGIK GKVSSRIHCG LQDSQKAGGA EDEQENEDDE EERDGEEEEE AEESNVEDLL ENNWNILQFL PQAASCQNYF LMIVSAYIVA VYHCMKDGLR RSAPGSTPVR RRGASQLSQE AEGAVGALPG MITFSQDYVA NELTQSFFTI TQKIQKKVTG SRNSTELSEM FPVLPGSHLL LNNPALEFIK YVCKVLSLDT NITNQVNKLN RDLLRLVDVG EFSEEAQFRD PCRSYVLPEV ICRSCNFCRD LDLCKDSSFS EDGAVLPQWL CSNCQAPYDS SAIEMTLVEV LQKKLVAFTL QDLVCLKCRG VKETSMPVYC SCAGDFALTI HTQVFMEQIG IFRNIAQHYG MSYLLETLEW LLQKNPQLGH

Characteristics: Human POLE full-length ORF ( AAH21559, 1 a.a. - 370 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

Target Details

Target: POLE (Polymerase (DNA Directed), Epsilon, Catalytic Subunit (POLE))

Alternative Name: POLE

Background: Full Gene Name: polymerase (DNA directed), epsilon
Synonyms: DKFZp434F222,FLJ21434,POLE1

Gene ID: 5426

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA