POLR1E Protein (AA 1-419) (GST tag)

Catalog No. ABIN1315665

This Recombinant POLR1E protein is expressed in Wheat germ and has been mentioned in 1 publication.

Quick Overview for POLR1E Protein (AA 1-419) (GST tag) (ABIN1315665)

Target: POLR1E (Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: ELISA, Western Blotting (WB), Affinity Purification (AP), Antibody Array (AA)

Product Details

Protein Characteristics: AA 1-419

Purification tag / Conjugate: This POLR1E protein is labelled with GST tag.

Purpose: PRAF1 (Human) Recombinant Protein (P01)

Sequence: MAAEVLPSAR WQYCGAPDGS QRAVLVQFSN GKLQSPGNMR FTLYENKDST NPRKRNQRIL AAETDRLSYV GNNFGTGALK CNTLCRHFVG ILNKTSGQME VYDAELFNMQ PLFSDVSVES ELALESQTKT YREKMDSCIE AFGTTKQKRA LNTRRMNRVG NESLNRAVAK AAETIIDTKG VTALVSDAIH NDLQDDSLYL PPCYDDAAKP EDVYKFEDLL SPAEYEALQS PSEAFRNVTS EEILKMIEEN SHCTFVIEAL KSLPSDVESR DRQARCIWFL DTLIKFRAHR VVKRKSALGP GVPHIINTKL LKHFTCLTYN NGRLRNLISD SMKAKITAYV IILALHIHDF QIDLTVLQRD LKLSEKRMME IAKAMRLKIS KRRVSVAAGS EEDHKLGTLS LPLPPAQTSD RLAKRRKIT

Characteristics: Human PRAF1 full-length ORF ( NP_071935.1, 1 a.a. - 419 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Kang, Shin: "Repression of rRNA transcription by PARIS contributes to Parkinson's disease." in: Neurobiology of disease, Vol. 73C, pp. 220-228, (2014) (PubMed).

Target Details

Target: POLR1E (Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E))

Alternative Name: POLR1E

Background: Full Gene Name: polymerase (RNA) I polypeptide E, 53 kDa
Synonyms: FLJ13390,FLJ13970,PAF53,PRAF1,RP11-405L18.3

Gene ID: 64425

NCBI Accession: NM_022490