Prosaposin Protein (PSAP) (AA 18-524) (GST tag)

Catalog No. ABIN1316504

This Recombinant Prosaposin protein is expressed in Wheat germ and has been mentioned in 2+ publications.

Quick Overview for Prosaposin Protein (PSAP) (AA 18-524) (GST tag) (ABIN1316504)

Target: Prosaposin (PSAP)

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)

Product Details

Protein Characteristics: AA 18-524

Purification tag / Conjugate: This Prosaposin protein is labelled with GST tag.

Purpose: PSAP (Human) Recombinant Protein (P01)

Sequence: PVLGLKECTR GSAVWCQNVK TASDCGAVKH CLQTVWNKPT VKSLPCDICK DVVTAAGDML KDNATEEEIL VYLEKTCDWL PKPNMSASCK EIVDSYLPVI LDIIKGEMSR PGEVCSALNL CESLQKHLAE LNHQKQLESN KIPELDMTEV VAPFMANIPL LLYPQDGPRS KPQPKDNGDV CQDCIQMVTD IQTAVRTNST FVQALVEHVK EECDRLGPGM ADICKNYISQ YSEIAIQMMM HMQPKEICAL VGFCDEVKEM PMQTLVPAKV ASKNVIPALE LVEPIKKHEV PAKSDVYCEV CEFLVKEVTK LIDNNKTEKE ILDAFDKMCS KLPKSLSEEC QEVVDTYGSS ILSILLEEVS PELVCSMLHL CSGTRLPALT VHVTQPKDGG FCEVCKKLVG YLDRNLEKNS TKQEILAALE KGCSFLPDPY QKQCDQFVAE YEPVLIEILV EVMDPSFVCL KIGACPSAHK PLLGTEKCIW GPSYWCQNTE TAAQCNAVEH CKRHVWN

Characteristics: Human PSAP full-length ORF ( AAH01503, 18 a.a. - 524 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Yamamoto-Tanaka, Motoyama, Miyai, Matsunaga, Matsuda, Tsuboi, Hibino: "Mesotrypsin and caspase-14 participate in prosaposin processing: potential relevance to epidermal permeability barrier formation." in: The Journal of biological chemistry, Vol. 289, Issue 29, pp. 20026-38, (2014) (PubMed).

Wu, Sun, Zou, Xu, Liu, Wang, Yun, Gu: "Prosaposin, a regulator of estrogen receptor alpha, promotes breast cancer growth." in: Cancer science, Vol. 103, Issue 10, pp. 1820-5, (2012) (PubMed).

Target Details

Target: Prosaposin (PSAP)

Alternative Name: PSAP

Background: Full Gene Name: prosaposin
Synonyms: FLJ00245,GLBA,MGC110993,SAP1

Gene ID: 5660

Pathways: Positive Regulation of Endopeptidase Activity