RAP1A Protein (AA 1-184) (GST tag)

Catalog No. ABIN1317390

Recombinant RAP1A protein expressed in Wheat germ and has been mentioned in 2+ publications.

Quick Overview for RAP1A Protein (AA 1-184) (GST tag) (ABIN1317390)

Target: RAP1A (RAP1A, Member of RAS Oncogene Family (RAP1A))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: Affinity Purification (AP), Antibody Array (AA), ELISA, Western Blotting (WB)

Product Details

Protein Characteristics: AA 1-184

Purification tag / Conjugate: This RAP1A protein is labelled with GST tag.

Purpose: RAP1A (Human) Recombinant Protein (P01)

Sequence: MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDCQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTEDVP MILVGNKCDL EDERVVGKEQ GQNLARQWCN CAFLESSAKS KINVNEIFYD LVRQINRKTP VEKKKPKKKS CLLL

Characteristics: Human RAP1A full-length ORF ( AAH14086.1, 1 a.a. - 184 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Suggested Applications*:elisa,antibody array,western blotting,affinity purification
*We do not guarantee this product working for suggested applications.
Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Branham, Bustos, De Blas, Rehmann, Zarelli, Treviño, Darszon, Mayorga, Tomes: "Epac activates the small G proteins Rap1 and Rab3A to achieve exocytosis." in: The Journal of biological chemistry, Vol. 284, Issue 37, pp. 24825-39, (2009) (PubMed).

Chu, Awasthi, White, Chrzanowska-Wodnicka, Malarkannan: "Rap1b regulates B cell development, homing, and T cell-dependent humoral immunity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 5, pp. 3373-83, (2008) (PubMed).

Target Details

Target: RAP1A (RAP1A, Member of RAS Oncogene Family (RAP1A))

Alternative Name: RAP1A

Background: Full Gene Name: RAP1A, member of RAS oncogene family
Synonyms: KREV-1,KREV1,RAP1,SMGP21

Gene ID: 5906

Pathways: TCR Signaling, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor