SIRT3 Protein (AA 1-399) (GST tag)

Catalog No. ABIN1320045

This Recombinant SIRT3 protein is expressed in Wheat germ and has been mentioned in 1 publication.

Quick Overview for SIRT3 Protein (AA 1-399) (GST tag) (ABIN1320045)

Target: SIRT3 (Sirtuin 3 (SIRT3))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)

Product Details

Protein Characteristics: AA 1-399

Purification tag / Conjugate: This SIRT3 protein is labelled with GST tag.

Purpose: SIRT3 (Human) Recombinant Protein (P01)

Sequence: MAFWGWRAAA ALRLWGRVVE RVEAGGGVGP FQACGCRLVL GGRDDVSAGL RGSHGARGEP LDPVRPLQRP PRPEVPRAFR RQPRAAAPSF FFSSIKGGRR SISFSVGASS VVGSGGSSDK GKLSLQDVAE LIRARACQRV VVMVGAGIST PSGIPDFRSP GSGLYSNLQQ YDLPYPEAIF ELPFFFHNPK PFFTLAKELY PGNYKPNVTH YFLRLLHDKG LLLRLYTQNI DGLERVSGIP ASKLVEAHGT FASATCTVCQ RPFPGEDIRA DVMADRVPRC PVCTGVVKPD IVFFGEPLPQ RFLLHVVDFP MADLLLILGT SLEVEPFASL TEAVRSSVPR LLINRDLVGP LAWHPRSRDV AQLGDVVHGV ESLVELLGWT EEMRDLVQRE TGKLDGPDK

Characteristics: Human SIRT3 full-length ORF ( AAH01042, 1 a.a. - 399 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Pacella-Ince, Zander-Fox, Lan: "Mitochondrial SIRT3 and its target glutamate dehydrogenase are altered in follicular cells of women with reduced ovarian reserve or advanced maternal age." in: Human reproduction (Oxford, England), Vol. 29, Issue 7, pp. 1490-9, (2014) (PubMed).

Target Details

Target: SIRT3 (Sirtuin 3 (SIRT3))

Alternative Name: SIRT3

Background: Full Gene Name: sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
Synonyms: SIR2L3

Gene ID: 23410