SIRT3 Protein (AA 5-257) (His tag)

Catalog No. ABIN6239915

Product Details

Target: SIRT3 (Sirtuin 3 (SIRT3))

Biological Activity: Active

Protein Characteristics: AA 5-257

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This SIRT3 protein is labelled with His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Characteristics: Tag location: N-terminal His Tag

Purity: > 90 %

Biological Activity Comment: Sirtuin 3 (SIRT3) also known as NAD-dependent deacetylase sirtuin-3 is a member of the mammalian sirtuin family of proteins. SIRT3 is a soluble protein located in the mitochondrial matrix, and contains a mitochondrial processing peptide at the N-terminus. It is a key regulator of succinate dehydrogenase (SDH), which catalyzes the oxidation of succinate to fumarate. Increased succinate concentrations and the specific G protein-coupled receptor 91 (GPR91) are involved in the activation of hepatic stellate cells (HSCs). SIRT3 agonists could help treat metabolic disorders such as obesity, metabolic syndrome and type 2 diabetes. Besides, Dihydrolipoyl Transacetylase (DLAT) has been identified as an interactor of SIRT3, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse SIRT3 and recombinant mouse DLAT. Briefly, SIRT3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to DLAT-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-SITR3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of SIRT3 and DLAT was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of SIRT3 with DLAT.

Application Details

Application Notes: Isoelectric Point: 6.4

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: SIRT3 (Sirtuin 3 (SIRT3))

Abstract: SIRT3 Products

Synonyms: zgc:158774 Protein, sirtuin Protein, SIR2L3 Protein, 2310003L23Rik Protein, AI848213 Protein, Sir2l3 Protein, sirtuin 3 Protein, sirt3 Protein, SIRT3 Protein, Sirt3 Protein

Background: Alternative Names: SIR2L3, Silent Mating Type Information Regulation 2 Homolog 3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, SIR2-like protein 3, Regulatory protein SIR2 homolog 3

Molecular Weight: 16&29kDa

UniProt: Q8R104