Topoisomerase I Protein (AA 692-765) (GST tag)

Catalog No. ABIN1323345

Recombinant Topoisomerase I protein expressed in Wheat germ.

Quick Overview for Topoisomerase I Protein (AA 692-765) (GST tag) (ABIN1323345)

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)

Product Details

Protein Characteristics: AA 692-765

Purification tag / Conjugate: This Topoisomerase I protein is labelled with GST tag.

Purpose: TOP1 (Human) Recombinant Protein (Q01)

Sequence: QRLEEQLMKL EVQATDREEN KQIALGTSKL NYLDPRITVA WCKKWGVPIE KIYNKTQREK FAWAIDMADE DYEF

Characteristics: Human TOP1 partial ORF ( NP_003277, 692 a.a. - 765 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

Target Details

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Alternative Name: TOP1

Background: Full Gene Name: topoisomerase (DNA) I
Synonyms: TOPI

Gene ID: 7150

NCBI Accession: NM_003286

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance