Topoisomerase I Protein (AA 2-767) (His tag)

Catalog No. ABIN1658449

Product Details

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Protein Type: Recombinant

Protein Characteristics: AA 2-767

Origin: Rat

Source: Yeast

Purification tag / Conjugate: This Topoisomerase I protein is labelled with His tag.

Application: ELISA

Sequence: SGDHLHNDS QIEADFRLND SHKHKDKHKD REHRHKEHKK DKDKDREKSK HSNSEHKDSE KKHKEKEKTK HKDGSSDKHK DKHKDRDKEK RKEEKIRAAG DAKIKKEKEN GFSSPPRIKD EPEDDGYFAP PKEDIKPLKR PRDEDDADYK PKKIKTEDIK KEKKRKLEEE EDGKLKKPKN KDKDKKVAEP DNKKKKAKKE EEQKWKWWEE ERYPEGIKWK FLEHKGPVFA PPYEPLPEGV KFYYDGKVMK LSPKAEEVAT FFAKMLDHEY TTKEIFRKNF FKDWRKEMTN DEKNTITNLS KCDFTQMSQY FKAQSEARKQ MSKEEKLKIK EENEKLLKEY GFCVMDNHRE RIANFKIEPP GLFRGRGNHP KMGMLKRRIM PEDIIINCSK DAKVPSPPPG HKWKEVRHDN KVTWLVSWTE NIQGSIKYIM LNPSSRIKGE KDWQKYETAR RLKKCVDKIR NQYREDWKSK EMKVRQRAVA LYFIDKLALR AGNEKEEGET ADTVGCCSLR VEHINLHPEL DGQEYVVEFD FPGKDSIRYY NKVPVEKRVF KNLQLFMENK QPEDDLFDRL NTGILNKHLQ DLMEGLTAKV FRTYNASITL QQQLKELTAP DENVPAKILS YNRANRAVAI LCNHQRAPPK TFEKSMMNLQ SKIDAKKDQL ADARKDLKSA KADAKVMKDA KTKKVVESKK KAVQRLEEQL MKLEVQATDR EENKQIALGT SKLNYLDPRI TVAWCKKWGV PIEKIYNKTQ REKFAWAIDM TDEDYEF

Specificity: Rattus norvegicus (Rat)

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Purity: > 90 %

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C, for extended storage, conserve at -20 °C or -80 °C.

Target Details

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Alternative Name: DNA topoisomerase 1 (Top1) (TOP1 Products)

Synonyms: CG6146 Protein, Dmel\\CG6146 Protein, TOP1 Protein, TopI Protein, Topo I Protein, TopoI Protein, dTopoI Protein, l(1)G0134 Protein, l(1)G0201 Protein, l(1)G0229 Protein, l(1)G0278 Protein, l(1)top1 Protein, top1 Protein, topo I Protein, topoI Protein, DNA topoisomerase 1 beta Protein, DNA topoisomerase I alpha Protein, MGO1 Protein, MGOUN 1 Protein, MTE17.1 Protein, MTE17_1 Protein, TOP1BETA Protein, TOPOISOMERASE 1 Protein, TOPI Protein, AI467334 Protein, D130064I21Rik Protein, Top-1 Protein, Ab2-086 Protein, topi Protein, Topoisomerase 1 Protein, DNA topoisomerase I Protein, DNA topoisomerase I alpha Protein, DNA topoisomerase 1 Protein, topoisomerase (DNA) I Protein, DNA topoisomerase I, gene 1 L homeolog Protein, Top1 Protein, top1 Protein, topA Protein, ZMO1193 Protein, MRAD2831_RS63095 Protein, Lferr_0016 Protein, PC1_2015 Protein, Za10_0136 Protein, Gbro_0575 Protein, Dd586_2108 Protein, Kvar_3044 Protein, Alvin_2069 Protein, Dacet_0849 Protein, Mrub_1615 Protein, Arnit_2962 Protein, Ndas_4953 Protein, Trad_2377 Protein, Acear_1653 Protein, Mahau_0844 Protein, Mesop_4109 Protein, Thein_1586 Protein, Theth_0241 Protein, TOP1ALPHA Protein, top-1 Protein, TOP1 Protein, top1.1.L Protein

Target Type: Viral Protein

Background: Recommended name: DNA topoisomerase 1.
EC= 5.99.1.2.
Alternative name(s): DNA topoisomerase I

UniProt: Q9WUL0

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance