Topoisomerase II alpha Protein (AA 1435-1531) (GST tag)
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- Target See all Topoisomerase II alpha (TOP2A) Proteins
- Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1435-1531
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This Topoisomerase II alpha protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- TOP2A (Human) Recombinant Protein (Q01)
- Sequence
- RAAPKGTKRD PALNSGVSQK PDPAKTKNRR KRKPSTSDDS DSNFEKIVSK AVTSKKSKGE SDDFHMDFDS AVAPRAKSVR AKKPIKYLEE SDEDDLF
- Characteristics
- Human TOP2A partial ORF ( NP_001058, 1435 a.a. - 1531 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Topoisomerase (DNA) II alpha 170kDa (TOP2A) (AA 1-1531) protein (Strep Tag)
Crystallography grade TOP2A Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Topoisomerase (DNA) II alpha 170kDa (TOP2A) (AA 1-1528) protein (Strep Tag)Crystallography grade TOP2A Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Topoisomerase (DNA) II alpha 170kDa (TOP2A) (AA 1354-1473) proteinTOP2A Origin: Human Host: Escherichia coli (E. coli) Recombinant >90 % as determined by SDS-PAGE.
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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- Target
- Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))
- Alternative Name
- TOP2A (TOP2A Products)
- Synonyms
- TOP2 Protein, TP2A Protein, TOP2A Protein, Top-2 Protein, TOP-2 Protein, F12B17.110 Protein, F12B17_110 Protein, thimet metalloendopeptidase 2 Protein, fc08c07 Protein, wu:fc08c07 Protein, DNA topoisomerase II alpha Protein, DNA topoisomerase II, alpha isozyme Protein, topoisomerase (DNA) II alpha Protein, Zincin-like metalloproteases family protein Protein, TOP2A Protein, Bm1_08705 Protein, Top2a Protein, AT5G10540 Protein, top2a Protein
- Background
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Full Gene Name: topoisomerase (DNA) II alpha 170 kDa
Synonyms: TOP2,TP2A - Gene ID
- 7153
- NCBI Accession
- NM_001067
- Pathways
- Cell Division Cycle, Mitotic G1-G1/S Phases
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