ANGPTL4 Protein (AA 1-410) (DYKDDDDK Tag)
Quick Overview for ANGPTL4 Protein (AA 1-410) (DYKDDDDK Tag) (ABIN1344082)
Target
See all ANGPTL4 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-410
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Purification tag / Conjugate
- This ANGPTL4 protein is labelled with DYKDDDDK Tag.
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Purpose
- ANGPTL4 (mouse) (rec.)
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Cross-Reactivity
- Mouse
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Characteristics
- Mouse ANGPTL4 (aa 1-410) is fused at the C-terminus to a FLAG®-tag.
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Sterility
- Sterile filtered
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Endotoxin Level
- <0.1EU/μg purified protein (LAL test).
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Biological Activity Comment
- Activates ERK phosphorylation in THP-1 cells.
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Angiopoietin-Like 4 (ANGPTL4) (AA 166-406) (Active) protein (His tag)
ANGPTL4 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Angiopoietin-Like 4 (ANGPTL4) (AA 26-406) protein (GST tag)ANGPTL4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Angiopoietin-Like 4 (ANGPTL4) (AA 164-406) (Active) protein (His tag)ANGPTL4 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Angiopoietin-Like 4 (ANGPTL4) protein (His tag)ANGPTL4 Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Angiopoietin-Like 4 (ANGPTL4) protein (Fc Tag)ANGPTL4 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Angiopoietin-Like 4 (ANGPTL4) (AA 168-410) (Active) protein (His tag)ANGPTL4 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Angiopoietin-Like 4 (ANGPTL4) (AA 26-130) protein (GST tag)ANGPTL4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- 0.2μm-filtered solution in PBS.
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Handling Advice
- After opening, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap.
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Storage
- 4 °C,-20 °C
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Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
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Expiry Date
- 6 months
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- ANGPTL4 (Angiopoietin-Like 4 (ANGPTL4))
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Alternative Name
- ANGPTL4
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Background
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Angiopoietin-like Protein 4, FIAF, Fasting-induced Adipose Factor, HFARP, Hepatic Fibrinogen/Angiopoietin-related Protein
ANGPTL4 (Angiopoietin-like protein 4) mainly expressed in endothelial cells (hypoxia-induced). Regulates angiogenesis and modulates tumorigenesis and directly regulates lipid, glucose, and energy metabolism. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. ANGPTL4 is a protein consisting of an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain (FLD). Both domains have distinct biological functions. The coiled-coil domain is responsible for the inhibitory effects on lipoprotein lipase (LPL) converting the active form of LPL into an inactive form, and the FLD domain mediates its antiangiogenic functions. The coiled coil and the FLD domains are separated by a short linker that can be cleaved after secretion. ANGPTL4 appears on the cell surface as the full-length form, where it can be released by heparin treatment. ANGPTL4 protein is then proteolytically cleaved by proprotein convertases (PCs), including furin, PC5/6, paired basic amino acid-cleaving enzyme 4, and PC7.
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Molecular Weight
- ~50kDa (SDS-PAGE)
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UniProt
- Q9Z1P8
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Pathways
- Regulation of Lipid Metabolism by PPARalpha
Target
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