IL-33 Protein (AA 109-266) (His tag)

Catalog No. ABIN1344108

This Recombinant IL-33 protein is expressed in Escherichia coli (E. coli).

Quick Overview for IL-33 Protein (AA 109-266) (His tag) (ABIN1344108)

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human, Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: >90 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 109-266

Purification tag / Conjugate: This IL-33 protein is labelled with His tag.

Purpose: IL-33 (mouse) (rec.) (His)

Specificity: Binds to mouse and human ST2.

Cross-Reactivity: Human, Mouse

Characteristics: Mouse IL-33 (aa 109-266) is fused at the C-terminus to a His-tag.

Sterility: Sterile filtered

Endotoxin Level: <1EU/μg purified protein (LAL test).

Biological Activity Comment: Activates mouse and human ST2-dependent NF-kappaB pathway.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.2μm-filtered solution in 55 mM TRIS-Cl, pH 8.2, containing 150 mM NaCl.

Handling Advice: After opening, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 2 years after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 24 months

Target Details

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Alternative Name: IL-33

Background:

Interleukin-33, IL-1F11, NF-HEV

Interleukin-33 (IL-33, HF-NEV, IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30 kDa human IL33 is converted by CASP1 to a 18 kDa protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1 (MAPK3)/ERK2 (MAPK1), p38 (MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.

Molecular Weight: ~20kDa (SDS-PAGE)

UniProt: Q2YEJ4

Pathways: Production of Molecular Mediator of Immune Response