Calreticulin Protein (CALR) (His tag)
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- Target See all Calreticulin (CALR) Proteins
- Calreticulin (CALR)
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Calreticulin protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purpose
- Calreticulin (human) (rec.) (His)
- Cross-Reactivity
- Human
- Characteristics
- Human calreticulin (aa18-417) is fused at the C-terminus to a His-tag.
- Purity
- >90 % (SDS-PAGE)
- Sterility
- Sterile filtered
- Endotoxin Level
- <1EU/μg purified protein (LAL test).
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Calreticulin (CALR) (AA 25-199) protein (His-IF2DI Tag)
CALR Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
Calreticulin (CALR) (AA 1-417) protein (GST tag)CALR Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Calreticulin (CALR) (AA 18-417) protein (His tag)CALR Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % by SDS - PAGE SDS
Calreticulin (CALR) (AA 18-417) protein (His tag)CALR Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- 0.2μm-filtered solution in 55 mM TRIS-Cl, pH 8.2, containing 150 mM NaCl.
- Handling Advice
- After opening, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
- Expiry Date
- 6 months
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- Target
- Calreticulin (CALR)
- Alternative Name
- Calreticulin (CALR Products)
- Background
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CRP55, Calregulin, Endoplasmic Reticulum Resident Protein 60, ERp60, grp60
Calreticulin is involved in regulation of intracellular Ca2+ homoeostasis and ER Ca2+ capacity. It constitutes together with calnexin and ERp57 the 'calreticulin/calnexin cycle' that is responsible for folding and quality control of newly synthesized glycoproteins. Calreticulin has been implicated to play a role in many biological systems, including functions inside and outside the ER, indicating that the protein is a multi-process molecule. Recently, Calreticulin was shown to enhance the merger of macrophages and tumor cells, increasing phagocytosis.
- Molecular Weight
- ~55kDa (SDS-PAGE)
- UniProt
- P27797
- Pathways
- Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response
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