Calreticulin Protein (CALR) (Fc Tag)
Quick Overview for Calreticulin Protein (CALR) (Fc Tag) (ABIN7317230)
Target
See all Calreticulin (CALR) ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This Calreticulin protein is labelled with Fc Tag.
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Purpose
- Recombinant Human CALR/Calreticulin Protein (Fc Tag)
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Sequence
- Met 1-Ala413
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Characteristics
- A DNA sequence encoding the human CALR (P27797) (Met1-Ala413) was expressed with the Fc region of human IgG1 at the C-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Calreticulin (CALR) (AA 18-417), (C-Term) protein (His tag)
CALR Origin: Human Host: Escherichia coli (E. coli) Recombinant >90 % (SDS-PAGE) SDS
Calreticulin (CALR) (AA 18-417) protein (His tag)CALR Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Calreticulin (CALR) (AA 25-199) protein (His-IF2DI Tag)CALR Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Calreticulin (CALR) (AA 18-417) protein (His tag)CALR Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % SDS
Calreticulin (CALR) (AA 1-417) protein (GST tag)CALR Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
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Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Expiry Date
- 12 months
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- Calreticulin (CALR)
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Alternative Name
- CALR/Calreticulin
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Background
- CRT,HEL-S-99n,RO,SSA,cC1qR,Calreticulin is a multifunctional protein. It acts as a main Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. Calreticulin binds Ca2+ ions (a second messenger in signal transduction), rendering it inactive. The Ca2+ is bound with low affinity, but high capacity, and can be released on a signal. Located in storage compartments associated with the endoplasmic reticulum, calreticulin also binds to misfolded proteins and prevents them from being exported from the endoplasmic reticulum to the golgi apparatus. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin reduces the binding of androgen receptor to its hormone-responsive DNA element and inhibits androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Therefore, calreticulin acts as a significant modulator of the regulation of gene transcription by nuclear hormone receptors.
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Molecular Weight
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Calculated MW: 73.0 kDa
Observed MW: 96&38 kDa
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Gene ID
- 811
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UniProt
- P27797
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Pathways
- Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response
Target
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