FAS Protein (AA 26-170) (Fc Tag)
Quick Overview for FAS Protein (AA 26-170) (Fc Tag) (ABIN1344148)
Target
See all FAS ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 26-170
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Purification tag / Conjugate
- This FAS protein is labelled with Fc Tag.
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Purpose
- Fas (human):Fc (human) (rec.)
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Specificity
- Binds human and mouse FasL.
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Cross-Reactivity
- Human, Mouse
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Characteristics
- The Fc portion of human IgG1 is fused to the C-terminus of human Fas (aa 26-170).
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Endotoxin Level
- <0.01EU/μg purified protein (LAL test).
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Biological Activity Comment
- Blocks the binding of FasL to Fas.
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TNF Receptor Superfamily, Member 6 (FAS) (AA 25-169) protein (His-DYKDDDDK-Strep II Tag)
FAS Origin: Human Host: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
TNF Receptor Superfamily, Member 6 (FAS) protein (His tag)FAS Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
TNF Receptor Superfamily, Member 6 (FAS) (Active) protein (His tag)FAS Origin: Mouse Host: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE Active
TNF Receptor Superfamily, Member 6 (FAS) (Active) protein (His tag)FAS Origin: Rat Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
TNF Receptor Superfamily, Member 6 (FAS) protein (Fc Tag)FAS Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
TNF Receptor Superfamily, Member 6 (FAS) (AA 1-335) protein (GST tag)FAS Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
TNF Receptor Superfamily, Member 6 (FAS) (AA 17-173) protein (His tag)FAS Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
TNF Receptor Superfamily, Member 6 (FAS) (AA 26-173) protein (Fc Tag)FAS Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
TNF Receptor Superfamily, Member 6 (FAS) (AA 26-173) (Active) protein (His tag)FAS Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 95 % SDS, AcA Active
TNF Receptor Superfamily, Member 6 (FAS) (AA 26-173) (Active) protein (Fc Tag)FAS Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute with 50 μL sterile water.
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Concentration
- 1 mg/mL
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Buffer
- Contains PBS.
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Handling Advice
- After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.
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Storage
- 4 °C,-20 °C
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Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
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Expiry Date
- 6 months
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- FAS (TNF Receptor Superfamily, Member 6 (FAS))
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Alternative Name
- Fas
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Background
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Tumor Necrosis Factor Receptor Superfamily Member 6, TNFRSF6, Apo-1 Antigen, CD95
FasL is a cytokine that binds to Fas/TNFRSF6, a receptor that transduces the apoptotic signal into cells. FasL is involved in cytotoxic T cell mediated apoptosis and in T cell development.
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Molecular Weight
- ~47kDa (SDS-PAGE).
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UniProt
- P25445
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Pathways
- p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
Target
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