TNFRSF10A Protein (AA 24-239) (Fc Tag)

Catalog No. ABIN1344260

This Recombinant TNFRSF10A protein is produced in HEK-293 Cells.

Quick Overview for TNFRSF10A Protein (AA 24-239) (Fc Tag) (ABIN1344260)

Target: TNFRSF10A (Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human, Mouse

Source: HEK-293 Cells

Application: SDS-PAGE (SDS)

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 24-239

Purification tag / Conjugate: This TNFRSF10A protein is labelled with Fc Tag.

Purpose: TRAIL-R1 (human):Fc (human) (rec.)

Specificity: Binds to human and mouse TRAIL.

Cross-Reactivity: Human, Mouse

Characteristics: The extracellular domain of human TRAIL-R1 (aa 24-239) is fused to the Fc portion of human IgG1.

Endotoxin Level: <0.01EU/μg purified protein (LAL test).

Biological Activity Comment: Blocks human or mouse TRAIL activity in a concentration of 1-10μg/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile water.

Concentration: 1 mg/mL

Buffer: Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: TNFRSF10A (Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))

Alternative Name: TRAIL-R1

Background:

DR4, TNFRSF 10A, APO2, CD261, TRAIL Receptor 1, TNF-related Apoptosis-inducing Ligand Receptor 1

TRAIL-R1 (DR4, CD261) is a receptor for the cytotoxic ligand TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappaB.

Molecular Weight: ~52kDa (SDS-PAGE)

UniProt: O00220

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity