CD40 Ligand Protein (CD40LG) (AA 18-111, AA 115-260) (DYKDDDDK Tag)

Catalog No. ABIN1344287

This Recombinant CD40 Ligand protein is produced in CHO Cells.

Quick Overview for CD40 Ligand Protein (CD40LG) (AA 18-111, AA 115-260) (DYKDDDDK Tag) (ABIN1344287)

Target: CD40 Ligand (CD40LG)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human, Mouse, Rat

Source: CHO Cells

Application: SDS-PAGE (SDS)

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 115-260, AA 18-111

Purification tag / Conjugate: This CD40 Ligand protein is labelled with DYKDDDDK Tag.

Purpose: CD40L (rat) (multimeric) (rec.)

Specificity: Binds to rat, human and mouse CD40.

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rat CD40L (aa 115-260) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.

Endotoxin Level: <0.02EU/μg purified protein (LAL test).

Biological Activity Comment: Induces B cell activation (as demonstrated by dose-dependent upregulation of CD86).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.1 mg/mL

Buffer: Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: CD40 Ligand (CD40LG)

Alternative Name: CD40L

Background:

MultimericCD40L™, ACRP30headless:CD40L, ACRP30headless:CD154, ACRP30headless:TNFSF5, ADIPOQ-CD40L

MultimericCD40L™ is a high activity construct in which two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively simulates the natural membrane-assisted aggregation of CD40L in vivo. It provides a simple and equally potent alternative to CD40L+enhancer combinations. MultimericCD40L™ has been shown to suppress alum-induced IL-1beta release and caspase-1 activation in a dose-, CD40- and time dependent manner, without affecting BMDM (Bone marrow-derived macrophages) viability. It also effectively suppressed the inflammasome function triggered by NLRP3 activators. The secretion of caspase-1 independent inflammatory mediators has been shown to be unaltered or even enhanced.

Molecular Weight: ~35-40kDa

UniProt: Q9Z2V2

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints