FASL Protein (AA 103-281, Soluble) (DYKDDDDK Tag)
Quick Overview for FASL Protein (AA 103-281, Soluble) (DYKDDDDK Tag) (ABIN1344396)
Target
See all FASL ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 103-281, Soluble
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Purification tag / Conjugate
- This FASL protein is labelled with DYKDDDDK Tag.
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Purpose
- FasL, Soluble (human) (rec.)
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Cross-Reactivity
- Human, Mouse
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Characteristics
- The extracellular domain of human FasL (aa 103-281) is fused at the N-terminus to a FLAG®-tag.
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Endotoxin Level
- <0.05EU/μg purified protein (LAL test).
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Biological Activity Comment
- Induces apoptosis of human Jurkat T cells at a concentration of <1ng/ml in the presence of 0.1 to 1μg/ml TNF Ligands Enhancer (Prod. No. AG-35B-0001). In the absence of TNF Ligands Enhancer, FasL is working at 50-100 fold higher concentrations.
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Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 134-281) (Active) protein (His tag)
FASL Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 134-281) (Active) protein (His tag)FASL Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Fas Ligand (TNF Superfamily, Member 6) (FASL) proteinFASL Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % pure SDS
Fas Ligand (TNF Superfamily, Member 6) (FASL) protein (His tag)FASL Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 99-330) protein (Fc Tag)FASL Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM
Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 1-281) protein (GST tag)FASL Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute with 100 μL sterile water.
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Concentration
- 0.1 mg/mL
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Buffer
- Contains PBS.
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Handling Advice
- After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.
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Storage
- 4 °C,-20 °C
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Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
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Expiry Date
- 6 months
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- FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
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Alternative Name
- FasL
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Background
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APO-1L, CD95L, CD178, TNFSF6, Fas Ligand
FasL is a cytokine that binds to TNFRSF6/Fas, a receptor that transduces the apoptotic signal into cells. Is involved in cytotoxic T cell mediated apoptosis and in T cell development.
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Molecular Weight
- ~33kDa (SDS-PAGE)
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UniProt
- P48023
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Pathways
- Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
Target
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