TNFSF18 Protein (AA 45-173, Extracellular Domain, Soluble) (DYKDDDDK Tag)

Catalog No. ABIN1344404

This Recombinant TNFSF18 protein is produced in HEK-293 Cells.

Quick Overview for TNFSF18 Protein (AA 45-173, Extracellular Domain, Soluble) (DYKDDDDK Tag) (ABIN1344404)

Target: TNFSF18 (Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Application: SDS-PAGE (SDS)

Purity: >90 % (SDS-PAGE)

Product Details

Protein Characteristics: Soluble, Extracellular Domain, AA 45-173

Purification tag / Conjugate: This TNFSF18 protein is labelled with DYKDDDDK Tag.

Purpose: GITRL, Soluble (mouse) (rec.)

Specificity: Binds to mouse GITR.

Cross-Reactivity: Mouse

Characteristics: The extracellular domain of mouse GITRL (aa 45-173) is fused at the N-terminus to a FLAG®-tag.

Sterility: Sterile filtered

Endotoxin Level: <0.1EU/μg purified protein (LAL test).

Biological Activity Comment: Induces GITR-mediated NF-kappaB activation in HEK 293 cells.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.2μm-filtered solution in 10 mM glycine, pH 10.0.

Handling Advice: After opening, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: TNFSF18 (Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18))

Alternative Name: GITRL

Background:

Glucocorticoid-induced TNF Receptor Ligand, AITRL, Activation-inducible TNF-related Ligand, TNFSF18

GITRL (Glucocorticoid-induced TNF receptor ligand) is expressed on dendritic cells (DC), monocytes, macrophages, B cells, activated T cells, endothelial cells, osteoclasts and various healthy non-lymphoid tissues (e.g. testis). GITRL is constitutively expressed and released as soluble form by solid tumors and various hematopoietic malignancies. GITRL causes differentiation of osteoclasts, activation of macrophages, but also alteration of carcinoma and leukemia cells and influences apoptosis. Binding to GITR is important in regulating T cell proliferation and TCR-mediated apoptosis. GITRL is implicated in development of autoimmune diseases and in the immune response against infectious pathogens and tumors.

Molecular Weight: ~22kDa (SDS-PAGE)

UniProt: Q7TS55