TNFSF4 Protein (AA 18-111, AA 52-199) (DYKDDDDK Tag)

Catalog No. ABIN1344408

This Recombinant TNFSF4 protein is expressed in HEK-293 Cells and has been mentioned in 1 publication.

Quick Overview for TNFSF4 Protein (AA 18-111, AA 52-199) (DYKDDDDK Tag) (ABIN1344408)

Target: TNFSF4 (Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human, Mouse

Source: HEK-293 Cells

Application: SDS-PAGE (SDS)

Purity: >90 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 18-111, AA 52-199

Purification tag / Conjugate: This TNFSF4 protein is labelled with DYKDDDDK Tag.

Purpose: OX40L (mouse) (multimeric) (rec.)

Specificity: Binds to human and mouse OX40 [CD134].

Cross-Reactivity: Human, Mouse

Characteristics: The extracellular domain of mouse OX40L (aa 52-199) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.

Endotoxin Level: <0.01EU/μg purified protein (LAL test).

Biological Activity Comment: Activates T cell proliferation.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.1 mg/mL

Buffer: Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

References

Mestas, Crampton, Hori, Hughes: "Endothelial cell co-stimulation through OX40 augments and prolongs T cell cytokine synthesis by stabilization of cytokine mRNA." in: International immunology, Vol. 17, Issue 6, pp. 737-47, (2005) (PubMed).

Target Details

Target: TNFSF4 (Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))

Alternative Name: OX40L

Background:

MultimericOX40L™, ACRP30headless:OX40L, ACRP30headless:CD252, ACRP30headless:CD134L

OX40L [CD252, CD134L] is a membrane-expressed cytokine that belongs to the TNF ligand family. It acts as costimulator through interaction with its receptor OX40 on T cells, stimulating T cell activation, proliferation and cytokine production. OX40L is expressed on antigen presenting cells including B cells, dendritic cells, mast cells and endothelium. MultimericOX40L is a high activity construct in which two trimeric OX40L ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively activates T cell proliferation.

Molecular Weight: ~40kDa (SDS-PAGE)

UniProt: P43488

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation, Cancer Immune Checkpoints