TNFSF13 Protein (AA 18-111, AA 88-232) (DYKDDDDK Tag)

Catalog No. ABIN1344410

This Recombinant TNFSF13 protein is produced in HEK-293 Cells. There is 1 publication available.

Quick Overview for TNFSF13 Protein (AA 18-111, AA 88-232) (DYKDDDDK Tag) (ABIN1344410)

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human, Mouse

Source: HEK-293 Cells

Application: SDS-PAGE (SDS)

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 18-111, AA 88-232

Purification tag / Conjugate: This TNFSF13 protein is labelled with DYKDDDDK Tag.

Purpose: APRIL (mouse) (multimeric) (rec.)

Brand: MegaAPRIL™

Specificity: Binds to human and mouse BCMA and TACI. Binds to proteoglycans.

Cross-Reactivity: Human, Mouse

Characteristics: The extracellular domain of mouse APRIL (aa 88-232) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.

Endotoxin Level: <0.02EU/μg purified protein (LAL test).

Biological Activity Comment: Stimulates B cell proliferation.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.1 mg/mL

Buffer: Contains PBS + 0.5 % Trehalose.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

References

Huard, Tran, Benkhoucha, Manzin-Lorenzi, Santiago-Raber: "Selective APRIL blockade delays systemic lupus erythematosus in mouse." in: PLoS ONE, Vol. 7, Issue 2, pp. e31837, (2012) (PubMed).

Target Details

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Alternative Name: APRIL

Background:

MultimericAPRIL™, ACRP30headless:APRIL, ACRP30headless:CD256, ACRP30headless:TNFSF13, ACRP30headless:A-proliferation-inducing Ligand:

APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth, is involved in monocyte/macrophage-mediated immunological processes and functions as an important survival factor for plasmablasts and bone marrow plasma cells. MultimericAPRIL™ is a high activity construct in which two trimeric APRIL ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively stimulates proliferation B cell. A basic amino acid sequence (QKQKKQ) close to the N-terminus of APRIL is required for binding to negatively charged sulfated glycosaminoglycan side chains of proteoglycans. Proteoglycans mediate binding of APRIL to tumor cells as well as primary lymphoid cells.

Molecular Weight: ~34kDa (SDS-PAGE)

UniProt: Q9D777

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response