Annexin V Protein (GST tag)

Catalog No. ABIN1344454

Recombinant Annexin V protein expressed in Escherichia coli (E. coli) and has been mentioned in 1 publication.

Quick Overview for Annexin V Protein (GST tag) (ABIN1344454)

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: >95 % (SDS-PAGE)

Product Details

Purification tag / Conjugate: This Annexin V protein is labelled with GST tag.

Purpose: Annexin V (human) (rec.)

Specificity: Binds to phosphatidylserine (PS).

Cross-Reactivity: Human

Characteristics: Recombinant human annexin V fused to a GST-tag.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 100 μL sterile water.

Concentration: 1 mg/mL

Buffer: In PBS.

Handling Advice: Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. Repeated freeze/thaw cycles (>3) may result in an adverse effect on the activity.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Reconstituted solutions can be kept at 4°C under sterile conditions for one week or should be frozen in aliquots at -20°C.

Expiry Date: 12 months

References

Morizono, Chen: "Role of phosphatidylserine receptors in enveloped virus infection." in: Journal of virology, Vol. 88, Issue 8, pp. 4275-90, (2014) (PubMed).

Target Details

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Alternative Name: Annexin V

Background:

Annexin A5, Endonexin II, Lipocortin V, Placental Anticoagulant Protein I, Calphobindin I, Vascular Anticoagulant-alpha

Annexin V is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Annexin V competes for phosphatidylserine binding sites (e.g. platelets) with prothrombin or coagulation factors and inhibis the activity of phospholipase A1. Antibodies directed against annexin V are found in patients with a disease called the antiphospholipid syndrome(APS), a thrombophilic disease associated with autoantibodies against phospholipid compounds. Annexin V is used as a probe in the "annexin V affinity assay" to detect cells that have expressed phosphatidylserine on the cell surface, a feature found in apoptosis and other forms of cell death.

Molecular Weight: ~52kDa (SDS-PAGE)

UniProt: P08758

Pathways: Apoptosis