ATF3 Protein (AA 1-181) (GST tag)

Catalog No. ABIN1345929

Recombinant ATF3 protein expressed in Wheat germ and has been mentioned in 3+ publications.

Quick Overview for ATF3 Protein (AA 1-181) (GST tag) (ABIN1345929)

Target: ATF3 (Activating Transcription Factor 3 (ATF3))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)

Product Details

Protein Characteristics: AA 1-181

Purification tag / Conjugate: This ATF3 protein is labelled with GST tag.

Purpose: ATF3 (Human) Recombinant Protein (P01)

Sequence: MMLQHPGQVS ASEVSASAIV PCLSPPGSLV FEDFANLTPF VKEELRFAIQ NKHLCHRMSS ALESVTVSDR PLGVSITKAE VAPEEDERKK RRRERNKIAA AKCRNKKKEK TECLQKESEK LESVNAELKA QIEELKNEKQ HLIYMLNLHR PTCIVRAQNG RTPEDERNLF IQQIKEGTLQ S

Characteristics: Human ATF3 full-length ORF ( AAH06322, 1 a.a. - 181 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Wang, Brennan, Gutierrez, Wang, Wang, Yang: "SUMOylation of ATF3 alters its transcriptional activity on regulation of TP53 gene." in: Journal of cellular biochemistry, Vol. 114, Issue 3, pp. 589-98, (2013) (PubMed).

Bembenek, Burkhardt, Ma, Li, Loke, Wu, Xu, Tayber, Xie, Li, Li: "Determination of complementary antibody pairs using protein A capture with the AlphaScreen assay format." in: Analytical biochemistry, Vol. 408, Issue 2, pp. 321-7, (2010) (PubMed).

Vigan, Neil: "Determinants of tetherin antagonism in the transmembrane domain of the human immunodeficiency virus type 1 Vpu protein." in: Journal of virology, Vol. 84, Issue 24, pp. 12958-70, (2010) (PubMed).

Target Details

Target: ATF3 (Activating Transcription Factor 3 (ATF3))

Alternative Name: ATF3

Background: Gene: activating transcription factor 3

Gene ID: 467

Pathways: Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response