ATP6AP1 Protein (AA 1-470) (GST tag)

Catalog No. ABIN1346087

This Recombinant ATP6AP1 protein is expressed in Wheat germ and has been mentioned in 1 publication.

Quick Overview for ATP6AP1 Protein (AA 1-470) (GST tag) (ABIN1346087)

Target: ATP6AP1 (ATPase, H+ Transporting, Lysosomal Accessory Protein 1 (ATP6AP1))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: Antibody Array (AA), Affinity Purification (AP), ELISA, Western Blotting (WB)

Product Details

Protein Characteristics: AA 1-470

Purification tag / Conjugate: This ATP6AP1 protein is labelled with GST tag.

Purpose: ATP6AP1 (Human) Recombinant Protein (P01)

Sequence: MMAAMATARV RMGPRCAQAL WRMPWLPVFL SLAAAAAAAA AEQQVPLVLW SSDRDLWAPA ADTHEGHITS DLQLSTYLDP ALELGPRNVL LFLQDKLSIE DFTAYGGVFG NKQDSAFSNL ENALDLAPSS LVLPAVDWYA VSTLTTYLQE KLGASPLHVD LATLRELKLN ASLPALLLIR LPYTASSGLM APREVLTGND EVIGQVLSTL KSEDVPYTAA LTAVRPSRVA RDVAVVAGGL GRQLLQKQPV SPVIHPPVSY NDTAPRILFW AQNFSVAYKD QWEDLTPLTF GVQELNLTGS FWNDSFARLS LTYERLFGTT VTFKFILANR LYPVSARHWF TMERLEVHSN GSVAYFNASQ VTGPSIYSFH CEYVSSLSKK GSLLVARTQP SPWQMMLQDF QIQAFNVMGE QFSYASDCAS FFSPGIWMGL LTSLFMLFIF TYGLHMILSL KTMDRFDDHK GPTISLTQIV

Characteristics: Human ATP6AP1 full-length ORF ( NP_001174.2, 1 a.a. - 470 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Lee, Hammerle, Andrews, Stokes, Mustelin, Silva, Black, Doedens: "Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels." in: The Journal of biological chemistry, Vol. 286, Issue 48, pp. 41530-8, (2011) (PubMed).

Target Details

Target: ATP6AP1 (ATPase, H+ Transporting, Lysosomal Accessory Protein 1 (ATP6AP1))

Alternative Name: ATP6AP1

Background: Full Gene Name: ATPase, H+ transporting, lysosomal accessory protein 1
Synonyms: 16A,ATP6IP1,ATP6S1,Ac45,CF2,MGC129781,VATPS1,XAP-3,XAP3

Gene ID: 537

NCBI Accession: NM_001183

Pathways: Proton Transport, SARS-CoV-2 Protein Interactome