CDK4 Protein (AA 1-303) (GST tag)

Catalog No. ABIN1348950

Recombinant CDK4 protein expressed in Wheat germ and has been mentioned in 1 publication.

Quick Overview for CDK4 Protein (AA 1-303) (GST tag) (ABIN1348950)

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)

Product Details

Protein Characteristics: AA 1-303

Purification tag / Conjugate: This CDK4 protein is labelled with GST tag.

Purpose: CDK4 (Human) Recombinant Protein (P02)

Sequence: MATSRYEPVA EIGVGAYGTV YKARDPHSGH FVALKSVRVP NGGGGGGGLP ISTVREVALL RRLEAFEHPN VVRLMDVCAT SRTDREIKVT LVFEHVDQDL RTYLDKAPPP GLPAETIKDL MRQFLRGLDF LHANCIVHRD LKPENILVTS GGTVKLADFG LARIYSYQMA LTPVVVTLWY RAPEVLLQST YATPVDMWSV GCIFAEMFRR KPLFCGNSEA DQLGKIFDLI GLPPEDDWPR DVSLPRGAFP PRGPRPVQSV VPEMEESGAQ LLLEMLTFNP HKRISAFRAL QHSYLHKDEG NPE

Characteristics: Human CDK4 full-length ORF ( AAH03644, 1 a.a. - 303 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Bockstaele, Kooken, Libert, Paternot, Dumont, de Launoit, Roger, Coulonval: "Regulated activating Thr172 phosphorylation of cyclin-dependent kinase 4(CDK4): its relationship with cyclins and CDK "inhibitors"." in: Molecular and cellular biology, Vol. 26, Issue 13, pp. 5070-85, (2006) (PubMed).

Target Details

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Alternative Name: CDK4

Background: Full Gene Name: cyclin-dependent kinase 4
Synonyms: CMM3,MGC14458,PSK-J3

Gene ID: 1019

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size