CDK4 Protein (GST tag)
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- Target See all CDK4 Proteins
- CDK4 (Cyclin-Dependent Kinase 4 (CDK4))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This CDK4 protein is labelled with GST tag.
- Purpose
- Recombinant Human CDK4 Protein (GST Tag)
- Sequence
- Met 1-Glu 303
- Characteristics
- A DNA sequence encoding the human CDK4 (NP_000066.1) (Met 1-Glu 303) was fused with the GST tag at the N-terminus.
- Purity
- > 80 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Top Product
- Discover our top product CDK4 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile 50 mM Tris, 100 mM NaCl, 10 % glycerol, 0.5 mM PMSF, pH 8.0
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- CDK4 (Cyclin-Dependent Kinase 4 (CDK4))
- Alternative Name
- CDK4 (CDK4 Products)
- Synonyms
- zgc:153726 Protein, CMM3 Protein, PSK-J3 Protein, Crk3 Protein, 8-6 Protein, CDK4 Protein, CDK4/6 Protein, CG5072 Protein, Cdk4/6 Protein, DmCdk4 Protein, Dmel\\CG5072 Protein, Pk53C Protein, Pk?7 Protein, cdk Protein, cdk4 Protein, cdk4/6 Protein, dCdk4 Protein, l(2)05428 Protein, l(2)0671 Protein, l(2)k06503 Protein, l(2)s4639 Protein, l(2)sh0671 Protein, xcdk4 Protein, cyclin-dependent kinase 4 Protein, cyclin dependent kinase 4 Protein, Cyclin-dependent kinase 4 Protein, cyclin-dependent kinase 4 L homeolog Protein, cdk4 Protein, CDK4 Protein, Cdk4 Protein, cdk4.L Protein
- Background
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Background: CDK4 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of CDK4 is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). CDK4 was shown to be responsible for the phosphorylation of retinoblastoma gene product. CDK4 is the ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. CDK4 has been shown to be mutated in some types of cancer, whilst a chromosomal rearrangement can lead to Cdk6 overexpression in lymphoma, leukemia and melanoma.
Synonym: CMM3,PSK-J3
- Molecular Weight
- 60 kDa
- NCBI Accession
- NP_000066
- Pathways
- Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size
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